Targeted metabolome profiling by dual-probe microdialysis sampling and treatment using Gardenia jasminoides for rats with type 2 diabetes.
Targeted metabolome profiling by dual-probe microdialysis sampling and treatment using Gardenia jasminoides for rats with type 2 diabetes.
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双探针微透析取样和栀子治疗 2 型糖尿病大鼠的靶向代谢组分析
DOI：
10.1038/s41598-017-10172-w
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发表时间：
2017-08-31
影响因子：
4.6
通讯作者：
Song F
中科院分区：
文献类型：
--
作者：
Wang L;Pi Z;Liu S;Liu Z;Song F
糖尿病会导致多种终末期器官并发症，包括糖尿病肾病。代谢组学为表征生物体液代谢变化提供了一种方法，但由于缺乏组织特异性代谢信息，针对糖尿病肾病的研究有限。一种用于采集完整内源性代谢物的微透析应用已被开发出来，它利用两个探针同时插入2型糖尿病大鼠的肾组织和颈静脉。对与2型糖尿病及其并发症密切相关的20种诊断生物标志物进行了全面定量分析。结果表明，糖尿病大鼠的氨基酸和核苷酸水平较低，这揭示了氨基酸以及嘌呤和嘧啶的代谢途径受到了干扰。利用质谱进行了靶向代谢组学研究，以寻找栀子（Gardenia jasminoides）治疗糖尿病的潜在治疗性生物标志物及相关代谢途径。结果表明，肾脏中的7种生物标志物和血液中的5种生物标志物与栀子有关。此外，栀子治疗后相关途径的显著扰动得到了调节，包括氨基酸代谢和嘌呤代谢。这些生物标志物和代谢途径为栀子治疗糖尿病及其并发症的分子机制提供了新的认识。
Diabetes causes a variety of end-stage organ complications, including diabetic nephropathy. Metabolomics offers an approach for characterizing biofluid metabolic changes, but studies focusing on diabetic nephropathy are limited due to the loss of tissue-specific metabolic information. A microdialysis application for the sampling of intact endogenous metabolites has been developed, utilizing two probes simultaneously inserted into the kidney tissues and jugular vein of rats with type 2 diabetes. The comprehensive and quantitative analysis of 20 diagnostic biomarkers closely realated to type 2 diabetes and its complications were performed. Results indicated that amino acid and nucleotide levels were lower in diabetic rats, revealing that the metabolic pathways of amino acid, as well as purine and pyrimidine, were disturbed. Targeted metabolomics using mass spectrometry was performed to find potential therapeutic biomarkers and related metabolic pathways of Gardenia jasminoides (G. jasminoides) for treating diabetes. Results suggested that seven biomarkers in the kidney and five biomarkers in the blood were related to G. jasminoides. In addition, the marked perturbations of pathways were regulated after treatment with G. jasminoides, including amino acid metabolism and purine metabolism. These biomarkers and metabolic pathways provided new understanding for molecular mechanisms of G. jasminoides for treating diabetes and its complications.
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影响因子：
16.6
作者：
Cudjoe, Erasmus;Bojko, Barbara;Pawliszyn, Janusz
通讯作者：
Pawliszyn, Janusz
影响因子：
--
作者：
Lin, Shuhai;Yang, Zhu;Cai, Zongwei
通讯作者：
Cai, Zongwei
阿卡波糖对糖尿病大鼠尿内源代谢物含量的影响
DOI：
10.1080/10826076.2013.850719
发表时间：
2014-01-01
影响因子：
1.3
作者：
Pi, Zifeng;Jiang, Hongyu;Liu, Zhiqiang
通讯作者：
Liu, Zhiqiang
影响因子：
4.4
作者：
Bohus, Eszter;Coen, Muireann;Nicholson, Jeremy K.
通讯作者：
Nicholson, Jeremy K.
影响因子：
7.1
作者：
Kalogeropoulou, Dionysia;LaFave, Laura;Nuttall, Frank Q.
通讯作者：
Nuttall, Frank Q.