The genome and developmental transcriptome of the strongylid nematode Haemonchus contortus.
The genome and developmental transcriptome of the strongylid nematode Haemonchus contortus.
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DOI：
10.1186/gb-2013-14-8-r89
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发表时间：
2013-08-28
期刊：
影响因子：
12.3
通讯作者：
Gasser RB
中科院分区：
文献类型：
--
作者：
Schwarz EM;Korhonen PK;Campbell BE;Young ND;Jex AR;Jabbar A;Hall RS;Mondal A;Howe AC;Pell J;Hofmann A;Boag PR;Zhu XQ;Gregory T;Loukas A;Williams BA;Antoshechkin I;Brown C;Sternberg PW;Gasser RB
理发师的杆虫，扭转血吸虫，是世界范围内小型反刍动物最重要的经济寄生虫之一。虽然这种寄生虫可以通过驱虫药控制，但对大多数常用药物的耐药性已成为一个普遍问题。我们提供了螺旋毛虫所有关键发育阶段的基因组和转录本草稿，以支持这种寄生虫和相关寄生虫的生物学和生物技术研究领域。螺旋藻的基因组草案大小为320 Mb，编码23,610个蛋白质编码基因。在基础水平上，我们阐明了整个生命周期中发生的转录变化，表征了寄生虫的基因沉默机制，并探索了参与发育、繁殖、宿主-寄生虫相互作用、免疫和疾病的分子。螺旋藻的分泌组特别富含与摄血活动和与宿主组织相互作用有关的多肽酶，并且参与了复杂的免疫反应。在应用层面上，我们预测药物目标并识别疫苗分子。螺旋藻基因组草案和发育转录组为科学界提供了广泛的基因组、遗传学、蛋白质组、代谢组、进化、生物学、生态学和流行病学研究的主要资源，并为生物技术成果，包括新的驱虫剂、疫苗和诊断测试奠定了坚实的基础。任何线虫的第一个基因组草图为我们快速了解最大线虫目之一的一种具有重要社会经济意义的寄生虫铺平了道路。
The barber's pole worm, Haemonchus contortus, is one of the most economically important parasites of small ruminants worldwide. Although this parasite can be controlled using anthelmintic drugs, resistance against most drugs in common use has become a widespread problem. We provide a draft of the genome and the transcriptomes of all key developmental stages of H. contortus to support biological and biotechnological research areas of this and related parasites. The draft genome of H. contortus is 320 Mb in size and encodes 23,610 protein-coding genes. On a fundamental level, we elucidate transcriptional alterations taking place throughout the life cycle, characterize the parasite's gene silencing machinery, and explore molecules involved in development, reproduction, host-parasite interactions, immunity, and disease. The secretome of H. contortus is particularly rich in peptidases linked to blood-feeding activity and interactions with host tissues, and a diverse array of molecules is involved in complex immune responses. On an applied level, we predict drug targets and identify vaccine molecules. The draft genome and developmental transcriptome of H. contortus provide a major resource to the scientific community for a wide range of genomic, genetic, proteomic, metabolomic, evolutionary, biological, ecological, and epidemiological investigations, and a solid foundation for biotechnological outcomes, including new anthelmintics, vaccines and diagnostic tests. This first draft genome of any strongylid nematode paves the way for a rapid acceleration in our understanding of a wide range of socioeconomically important parasites of one of the largest nematode orders.
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影响因子：
14.9
作者：
NCBI Resource Coordinators
通讯作者：
NCBI Resource Coordinators
影响因子：
2.7
作者：
Campbell, Bronwyn E.;Tarleton, Mark;Gasser, Robin B.
通讯作者：
Gasser, Robin B.
影响因子：
30.8
作者：
Dieterich, Christoph;Clifton, Sandra W.;Schuster, Lisa N.;Chinwalla, Asif;Delehaunty, Kimberly;Dinkelacker, Iris;Fulton, Lucinda;Fulton, Robert;Godfrey, Jennifer;Minx, Pat;Mitreva, Makedonka;Roeseler, Waltraud;Tian, Huiyu;Witte, Hanh;Yang, Shiaw-Pyng;Wilson, Richard K.;Sommer, Ralf J.
通讯作者：
Sommer, Ralf J.
影响因子：
2.6
作者：
Datu, Bennett J. D.;Loukas, Alex;Gasser, Robin B.
通讯作者：
Gasser, Robin B.
影响因子：
14.9
作者：
Cock PJ;Fields CJ;Goto N;Heuer ML;Rice PM
通讯作者：
Rice PM