Architecture of the outer-membrane core complex from a conjugative type IV secretion system.
Architecture of the outer-membrane core complex from a conjugative type IV secretion system.
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DOI：
10.1038/s41467-021-27178-8
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发表时间：
2021-11-25
影响因子：
16.6
通讯作者：
Costa TRD
中科院分区：
文献类型：
--
作者：
Amin H;Ilangovan A;Costa TRD
接合是细菌在细菌种群中传播抗生素耐药基因的最重要的过程之一。细菌间DNA转移需要一个由内膜复合体(IMC)、外膜核心复合体(OMCC)和结合菌毛组成的称为4型分泌系统(T4SS)的双跨膜纳米机器。标志性的F质粒编码的T4SS几十年来一直是理解接合的核心，然而其结构的原子细节尚不清楚。在这里，我们报道了由来自大肠杆菌的pED208质粒编码的完整的接合的OMCC的结构，并用冰冻电子显微镜以3.3 á的分辨率进行了解析。这种2.1丙二醛复合体具有两个径向同心环的独特排列，每个环具有不同的对称性，最终导致与其他OMCC相比，在蛋白质化学计量和灵活性方面存在显著差异。我们的结构表明，F-OMCC是一个高度动态的复合体，意味着在接合过程中毛发的伸展和收缩。DNA在两个细菌细胞之间的转移是由T4型分泌系统(T4SS)介导的。在这里，作者报告了一个完整的T4SS外膜核心复合体(OMCC)的结构，分别揭示了其中心内环区和外围外环区的明显C17和C13对称性。
Conjugation is one of the most important processes that bacteria utilize to spread antibiotic resistance genes among bacterial populations. Interbacterial DNA transfer requires a large double membrane-spanning nanomachine called the type 4 secretion system (T4SS) made up of the inner-membrane complex (IMC), the outer-membrane core complex (OMCC) and the conjugative pilus. The iconic F plasmid-encoded T4SS has been central in understanding conjugation for several decades, however atomic details of its structure are not known. Here, we report the structure of a complete conjugative OMCC encoded by the pED208 plasmid from E. coli, solved by cryo-electron microscopy at 3.3 Å resolution. This 2.1 MDa complex has a unique arrangement with two radial concentric rings, each having a different symmetry eventually contributing to remarkable differences in protein stoichiometry and flexibility in comparison to other OMCCs. Our structure suggests that F-OMCC is a highly dynamic complex, with implications for pilus extension and retraction during conjugation. DNA transfer between two bacterial cells is mediated by the conjugative type 4 secretion systems (T4SSs). Here, the authors report the structure of a complete T4SS outer-membrane core complex (OMCC), revealing distinct C17 and C13 symmetries of its central inner and peripheral outer ring regions, respectively.
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影响因子：
3.1
作者：
Isaac DT;Isberg R
通讯作者：
Isberg R
影响因子：
64.5
作者：
Costa TRD;Ilangovan A;Ukleja M;Redzej A;Santini JM;Smith TK;Egelman EH;Waksman G
通讯作者：
Waksman G
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH
影响因子：
3.2
作者：
Baron, C;Thorstenson, YR;Zambryski, PC
通讯作者：
Zambryski, PC
DOI：
10.1107/s0907444909042073
发表时间：
2010-01
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Chen VB;Arendall WB 3rd;Headd JJ;Keedy DA;Immormino RM;Kapral GJ;Murray LW;Richardson JS;Richardson DC
通讯作者：
Richardson DC