Increasing cellular uptake of mesoporous silica nanoparticles in human embryonic kidney cell line 293T cells by using Lipofectamine 2000.
Increasing cellular uptake of mesoporous silica nanoparticles in human embryonic kidney cell line 293T cells by using Lipofectamine 2000.
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使用 Lipofectamine 2000 增加人胚胎肾细胞系 293T 细胞对介孔二氧化硅纳米粒子的细胞摄取。
DOI：
10.1166/jbn.2013.1691
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发表时间：
2013-09
影响因子：
2.9
通讯作者：
Wang Xin
中科院分区：
文献类型：
--
作者：
Lu Guangming;Teng Zhaogang;Tian Ying;Fang Tian;Ma Jie;Sun Jin;Zhu Feipeng;Wu Jinrong;Wang Xin
介孔二氧化硅纳米颗粒(MSN)是一种理想的纳米载体，近年来在药物、基因和蛋白质递送等重要生物应用中得到了广泛的关注。内吞作用的有效性对MSN的生物学功能有很大影响。在本研究中，我们研究了脂质体2000阳离子脂质体对MSN细胞摄取的影响，以及阳离子脂质体与MSN在体内外的细胞毒性。因此，我们合成了平均粒径为130 nm、表面带负电荷的介孔二氧化硅纳米颗粒，并对其进行了表征。用透射电子显微镜和电感耦合等离子体分析技术研究了脂联胺2000对人胚肾细胞系293T细胞摄取MSN的可能作用。通过细胞凋亡率、细胞存活率测定和体内组织学观察等方法检测脂质体与MSN的联合作用。与对照组(P<；0.001)相比，脂质体能显著提高MSN对人胚胎肾293T细胞的吞噬效率(P<；)。脂质体2000与MSN联用在体内外均无明显的细胞毒性。我们的研究结果表明，阳离子脂质体脂质体2000具有显著增加人肾293T细胞对表面带负电荷的MSN的摄取能力，且无明显毒性。
Mesoporous silica nanoparticles (MSNs) are ideal nanocarriers that have recently gained attention in important bioapplications such as drug, gene, and protein delivery. The efficacy of endocytosis greatly affects the biological functions of MSNs. In the present study, we investigated the effect of cationic liposomes of Lipofectamine 2000 on cellular uptake of MSNs and the cytotoxicity of cationic liposomes combining with MSNs both in vitro and in vivo. Therefore, mesoporous silica nanoparticles with an average diameter of 130 nm and negative surface charge were synthesized and characterized. The possible role of Lipofectamine 2000 in cellular uptake of MSNs was evaluated in human embryonic kidney cell line 293T cells by transmission electron microscopy (TEM) and with inductively coupled plasma (ICP) analysis. The toxicities of liposomes combining with MSNs were tested in vitro via cell apoptosis assay and MTT cell viability assay, and in vivo by histological examination of six organs of mice after intravenous injection. The endocytosis efficiency of MSNs in human embryonic kidney 293T cells was greatly increased using Lipofectamine 2000 compared with controls (P < 0.001). No apparent in vitro or in vivo cytotoxicity was found for Lipofectamine 2000 combining with MSNs. Our data indicate that cationic liposomes of Lipofectamine 2000 has the potential to greatly increase cellular uptake of MSNs with negative surface charge in human renal 293T cells without apparent toxicity.
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影响因子：
17.1
作者：
Ashley CE;Carnes EC;Epler KE;Padilla DP;Phillips GK;Castillo RE;Wilkinson DC;Wilkinson BS;Burgard CA;Kalinich RM;Townson JL;Chackerian B;Willman CL;Peabody DS;Wharton W;Brinker CJ
通讯作者：
Brinker CJ
影响因子：
7.4
作者：
Huo Q;Litherland SA;Sullivan S;Hallquist H;Decker DA;Rivera-Ramirez I
通讯作者：
Rivera-Ramirez I
DOI：
10.1016/j.nano.2011.08.003
发表时间：
2012-05
期刊：
Nanomedicine : nanotechnology, biology, and medicine
影响因子：
--
作者：
H. Townley;E. Rapa;G. Wakefield;P. Dobson
通讯作者：
H. Townley;E. Rapa;G. Wakefield;P. Dobson
影响因子：
16.6
作者：
Lee, In-Hyun;Kwon, Ho-Keun;Jon, Sangyong
通讯作者：
Jon, Sangyong
影响因子：
3.8
作者：
GRAHAM, FL;SMILEY, J;NAIRN, R
通讯作者：
NAIRN, R