Cervical prephrenic interneurons in the normal and lesioned spinal cord of the adult rat.
Cervical prephrenic interneurons in the normal and lesioned spinal cord of the adult rat.
复制标题
成年大鼠正常和病变的脊髓中的宫颈预性中间神经元。
DOI：
10.1002/cne.21864
复制
发表时间：
2008-12-10
影响因子：
2.5
通讯作者：
Reier, Paul J.
中科院分区：
文献类型：
--
作者：
Lane, Michael A.;White, Todd E.;Coutts, Marcella A.;Jones, Alex L.;Sandhu, Milapjit S.;Bloom, David C.;Bolser, Donald C.;Yates, Bill J.;Fuller, David D.;Reier, Paul J.
关键词：
虽然单突触球脊髓向膈运动神经元的投射已被广泛描述，但对成年大鼠脊髓中膈前运动神经元的组织知之甚少。由于中间神经元可能在脊髓损伤后的正常呼吸和恢复中发挥重要作用，本研究采用顺行和跨神经元逆行示踪来研究它们的分布和突触关系。用伪狂犬病毒对膈运动神经元池进行单侧一级标记，显示大量二级、双侧分布的颈间神经元主要分布在背角和中央椎管周围。经神经元示踪和顺行示踪显示腹侧呼吸柱投射到膈前中间神经元，提示髓质神经元和膈核之间存在一些本体脊髓传递。假狂犬病毒重组的双标记研究也显示膈前中间神经元与对侧膈或肋间运动神经元池整合。神经间假狂犬病毒标记模式的稳定性后，侧宫颈半切处理。除了在中央管水平上感染的对侧中间神经元较少外，半切后两周（即损伤后最早恢复膈活动的时间）膈相关中间神经元的数量和分布没有明显改变。这些结果证明了膈相关中间神经元的异质性。它们在颈椎半切后的连通性和相对稳定性引发了对正常和损伤后调节膈功能的潜在多种作用的猜测。
While monosynaptic bulbospinal projections to phrenic motoneurons have been extensively described, little is known about the organization of phrenic premotor neurons in the adult rat spinal cord. As interneurons may play an important role in normal breathing and recovery following spinal cord injury, the present study has used anterograde and transneuronal retrograde tracing to study their distribution and synaptic relations. Exclusive unilateral, first-order labeling of the phrenic motoneuron pool with pseudorabies virus demonstrated a substantial number of second-order, bilaterally-distributed cervical interneurons predominantly in the dorsal horn and around the central canal. Combined transneuronal and anterograde tracing revealed ventral respiratory column projections to pre-phrenic interneurons suggesting some propriospinal relays exist between medullary neurons and the phrenic nucleus. Dual-labeling studies with pseudorabies virus recombinants also showed pre-phrenic interneurons integrated with either contralateral phrenic or intercostal motoneuron pools. The stability of interneuronal pseudorabies virus labeling patterns following lateral cervical hemisection was then addressed. Except for fewer infected contralateral interneurons at the level of the central canal, the number and distribution of phrenic-associated interneurons was not significantly altered two weeks post-hemisection (i.e. when the earliest post-injury recovery of phrenic activity has been reported). These results demonstrate a heterogeneous population of phrenic-related interneurons. Their connectivity and relative stability after cervical hemisection raises speculation for potentially diverse roles in modulating phrenic function normally and post-injury.
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