Evaluation of the Illumigene Malaria LAMP: A Robust Molecular Diagnostic Tool for Malaria Parasites.
Evaluation of the Illumigene Malaria LAMP: A Robust Molecular Diagnostic Tool for Malaria Parasites.
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DOI：
10.1038/srep36808
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发表时间：
2016-11-09
影响因子：
4.6
通讯作者：
Ndiaye D
中科院分区：
文献类型：
--
作者：
Lucchi NW;Gaye M;Diallo MA;Goldman IF;Ljolje D;Deme AB;Badiane A;Ndiaye YD;Barnwell JW;Udhayakumar V;Ndiaye D
等温核酸扩增测定，如环介导等温扩增（LAMP），非常适合现场使用，因为它们不需要热循环仪来扩增DNA。为了进一步促进远程环境中LAMP测定的使用，需要更简单的样品制备方法和冻干试剂。使用两种样品制备工作流程（简单过滤制备（SFP））和重力驱动过滤制备（GFP））和预分配的冻干试剂评估商业疟疾LAMP测定（Illumigene Malaria LAMP）的性能。实验室和临床样本在塞内加尔的现场实验室进行检测，结果在美国的参考实验室独立确认。Illumigene Malaria LAMP检测试剂盒在临床实验室中易于实施，结果与实时PCR参考检测试剂盒相似，检测限≤2.0个寄生虫/μl，具体取决于所用的样本制备方法。该检测方法以简单的低技术形式可靠地检测疟原虫属寄生虫，为疟疾诊断提供了更复杂的分子检测的替代方案。
Isothermal nucleic acid amplification assays such as the loop mediated isothermal amplification (LAMP), are well suited for field use as they do not require thermal cyclers to amplify the DNA. To further facilitate the use of LAMP assays in remote settings, simpler sample preparation methods and lyophilized reagents are required. The performance of a commercial malaria LAMP assay (Illumigene Malaria LAMP) was evaluated using two sample preparation workflows (simple filtration prep (SFP)) and gravity-driven filtration prep (GFP)) and pre-dispensed lyophilized reagents. Laboratory and clinical samples were tested in a field laboratory in Senegal and the results independently confirmed in a reference laboratory in the U.S.A. The Illumigene Malaria LAMP assay was easily implemented in the clinical laboratory and gave similar results to a real-time PCR reference test with limits of detection of ≤2.0 parasites/μl depending on the sample preparation method used. This assay reliably detected Plasmodium sp. parasites in a simple low-tech format, providing a much needed alternative to the more complex molecular tests for malaria diagnosis.
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DOI：
10.1093/infdis/jit183
发表时间：
2013-08-15
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Polley SD;González IJ;Mohamed D;Daly R;Bowers K;Watson J;Mewse E;Armstrong M;Gray C;Perkins MD;Bell D;Kanda H;Tomita N;Kubota Y;Mori Y;Chiodini PL;Sutherland CJ
通讯作者：
Sutherland CJ
在疟疾流行环境中对疟疾寄生虫血症的高度敏感性检测：在乌干达偏远诊所中新的环路介导的等温扩增试剂盒的性能。
DOI：
10.1093/infdis/jit184
发表时间：
2013-08-15
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Hopkins H;González IJ;Polley SD;Angutoko P;Ategeka J;Asiimwe C;Agaba B;Kyabayinze DJ;Sutherland CJ;Perkins MD;Bell D
通讯作者：
Bell D
影响因子：
3.7
作者：
Lucchi NW;Demas A;Narayanan J;Sumari D;Kabanywanyi A;Kachur SP;Barnwell JW;Udhayakumar V
通讯作者：
Udhayakumar V
影响因子：
3
作者：
Sema M;Alemu A;Bayih AG;Getie S;Getnet G;Guelig D;Burton R;LaBarre P;Pillai DR
通讯作者：
Pillai DR
亚显微疟疾寄生虫携带：基于聚合酶链反应的方法的重复性如何？
影响因子：
2.8
作者：
Costa DC;Madureira AP;Amaral LC;Sanchez BA;Gomes LT;Fontes CJ;Limongi JE;Brito CF;Carvalho LH
通讯作者：
Carvalho LH