Seven Years at High Salinity-Experimental Evolution of the Extremely Halotolerant Black Yeast Hortaea werneckii.
Seven Years at High Salinity-Experimental Evolution of the Extremely Halotolerant Black Yeast Hortaea werneckii.
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DOI：
10.3390/jof7090723
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发表时间：
2021-09-04
期刊：
影响因子：
--
通讯作者：
Gunde-Cimerman N
中科院分区：
文献类型：
--
作者：
Gostinčar C;Stajich JE;Kejžar A;Sinha S;Nislow C;Lenassi M;Gunde-Cimerman N
暴露于极端条件下的微生物的实验进化可以提供对细胞适应压力的洞察。通常情况下，压力敏感的物种暴露在压力下许多代，然后检查其耐压力的改善。相比之下，当从已经具有胁迫耐受性的祖细胞开始时，进一步改进的空间可能较小，它仍然能够调整其细胞机制以增加极端耐受性，这可能是以在非极端条件下性能较差为代价的。为了研究这些可能性，一株极端耐盐的黑酵母Hortaea werneckii在含有4.3 M NaCl的培养基中生长了7年以上，经过至少800代。虽然这个盐度远高于该物种的最适盐度（0.8-1.7 M），但进化的H. werneckii没有改变，在没有盐或在高浓度的NaCl，KCl，山梨醇，或甘油。在实验进化过程中，其他表型特征确实发生了变化，包括进化菌株中的多细胞链减少，细胞明显变窄，对卡泊芬净的抗性增加，以及黑化改变。全基因组测序揭示了在二倍体H.韦内克ii。在非整倍体区域观察到几个基因组的显着过度表达。两者合计，这些变化表明，在极端盐度下的长期生长导致细胞壁和形态，信号通路和磷酸戊糖循环的改变。虽然目前这些变化的适应价值的证据有限，但它们为未来的真菌耐盐性研究提供了有希望的起点。
The experimental evolution of microorganisms exposed to extreme conditions can provide insight into cellular adaptation to stress. Typically, stress-sensitive species are exposed to stress over many generations and then examined for improvements in their stress tolerance. In contrast, when starting with an already stress-tolerant progenitor there may be less room for further improvement, it may still be able to tweak its cellular machinery to increase extremotolerance, perhaps at the cost of poorer performance under non-extreme conditions. To investigate these possibilities, a strain of extremely halotolerant black yeast Hortaea werneckii was grown for over seven years through at least 800 generations in a medium containing 4.3 M NaCl. Although this salinity is well above the optimum (0.8–1.7 M) for the species, the growth rate of the evolved H. werneckii did not change in the absence of salt or at high concentrations of NaCl, KCl, sorbitol, or glycerol. Other phenotypic traits did change during the course of the experimental evolution, including fewer multicellular chains in the evolved strains, significantly narrower cells, increased resistance to caspofungin, and altered melanisation. Whole-genome sequencing revealed the occurrence of multiple aneuploidies during the experimental evolution of the otherwise diploid H. werneckii. A significant overrepresentation of several gene groups was observed in aneuploid regions. Taken together, these changes suggest that long-term growth at extreme salinity led to alterations in cell wall and morphology, signalling pathways, and the pentose phosphate cycle. Although there is currently limited evidence for the adaptive value of these changes, they offer promising starting points for future studies of fungal halotolerance.
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影响因子：
7.7
作者：
Johnson MS;Gopalakrishnan S;Goyal J;Dillingham ME;Bakerlee CW;Humphrey PT;Jagdish T;Jerison ER;Kosheleva K;Lawrence KR;Min J;Moulana A;Phillips AM;Piper JC;Purkanti R;Rego-Costa A;McDonald MJ;Nguyen Ba AN;Desai MM
通讯作者：
Desai MM
影响因子：
4.5
作者：
Gerstein AC;Chun HJ;Grant A;Otto SP
通讯作者：
Otto SP
影响因子：
4.4
作者：
Gostinčar C;Stajich JE;Zupančič J;Zalar P;Gunde-Cimerman N
通讯作者：
Gunde-Cimerman N
影响因子：
6.4
作者：
Cesar, Spencer;Anjur-Dietrich, Maya;Huang, Kerwyn Casey
通讯作者：
Huang, Kerwyn Casey
DOI：
10.1073/pnas.1807796115
发表时间：
2018-11-27
影响因子：
11.1
作者：
Hou J;Shi X;Chen C;Islam MS;Johnson AF;Kanno T;Huettel B;Yen MR;Hsu FM;Ji T;Chen PY;Matzke M;Matzke AJM;Cheng J;Birchler JA
通讯作者：
Birchler JA