Genomic evidence for intraspecific hybridization in a clonal and extremely halotolerant yeast.
Genomic evidence for intraspecific hybridization in a clonal and extremely halotolerant yeast.
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DOI：
10.1186/s12864-018-4751-5
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发表时间：
2018-05-15
期刊：
影响因子：
4.4
通讯作者：
Gunde-Cimerman N
中科院分区：
文献类型：
--
作者：
Gostinčar C;Stajich JE;Zupančič J;Zalar P;Gunde-Cimerman N
黑酵母Hortaea werneckii（Dothideomycetes，Ascomycota）是最极端的耐盐真菌之一，能够在接近饱和的NaCl浓度下生长。虽然dothideomycetous真菌是典型的单倍体，参考H。Werneckii菌株具有由具有高水平杂合性的两个亚基因组组成的二倍体基因组。为了解释H. Werneckii二倍体基因组我们在此报告了从不同生境和地理位置分离的11个菌株的基因组测序。9个二倍体和两个单倍体菌株的比较表明，参考基因组可能是由两个单倍体之间的杂交，而不是由核内复制如前所述。结果还支持额外的杂交事件在进化史上的调查菌株，但交换的遗传物质的物种，否则似乎是罕见的。这种不寻常的繁殖和极端耐受性之间的可能联系。werneckii仍有待调查。H. Werneckii似乎能够形成持久的单倍体以及二倍体菌株，能够在相对杂合的单倍体之间偶尔杂交，但在其它方面限于克隆繁殖。报道的数据和首次鉴定的单倍体H. werneckii菌株将该种建立为研究在不受频繁遗传重组干扰的极端耐受系统中倍性和杂交的影响的良好模型。本文的在线版本（10.1186/s12864-018-4751-5）包含补充材料，可供授权用户使用。
The black yeast Hortaea werneckii (Dothideomycetes, Ascomycota) is one of the most extremely halotolerant fungi, capable of growth at NaCl concentrations close to saturation. Although dothideomycetous fungi are typically haploid, the reference H. werneckii strain has a diploid genome consisting of two subgenomes with a high level of heterozygosity. In order to explain the origin of the H. werneckii diploid genome we here report the genome sequencing of eleven strains isolated from different habitats and geographic locations. Comparison of nine diploid and two haploid strains showed that the reference genome was likely formed by hybridization between two haploids and not by endoreduplication as suggested previously. Results also support additional hybridization events in the evolutionary history of investigated strains, however exchange of genetic material in the species otherwise appears to be rare. Possible links between such unusual reproduction and the extremotolerance of H. werneckii remain to be investigated. H. werneckii appears to be able to form persistent haploid as well as diploid strains, is capable of occasional hybridization between relatively heterozygous haploids, but is otherwise limited to clonal reproduction. The reported data and the first identification of haploid H. werneckii strains establish this species as a good model for studying the effects of ploidy and hybridization in an extremotolerant system unperturbed by frequent genetic recombination. The online version of this article (10.1186/s12864-018-4751-5) contains supplementary material, which is available to authorized users.
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影响因子：
12.3
作者：
Hane JK;Rouxel T;Howlett BJ;Kema GH;Goodwin SB;Oliver RP
通讯作者：
Oliver RP
DOI：
10.1534/g3.111.000745
发表时间：
2012-02
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Louis VL;Despons L;Friedrich A;Martin T;Durrens P;Casarégola S;Neuvéglise C;Fairhead C;Marck C;Cruz JA;Straub ML;Kugler V;Sacerdot C;Uzunov Z;Thierry A;Weiss S;Bleykasten C;De Montigny J;Jacques N;Jung P;Lemaire M;Mallet S;Morel G;Richard GF;Sarkar A;Savel G;Schacherer J;Seret ML;Talla E;Samson G;Jubin C;Poulain J;Vacherie B;Barbe V;Pelletier E;Sherman DJ;Westhof E;Weissenbach J;Baret PV;Wincker P;Gaillardin C;Dujon B;Souciet JL
通讯作者：
Souciet JL
影响因子：
5.8
作者：
Simao, Felipe A.;Waterhouse, Robert M.;Zdobnov, Evgeny M.
通讯作者：
Zdobnov, Evgeny M.
DOI：
10.1046/j.1471-8278.2000.00014.x
发表时间：
2001-03-01
期刊：
MOLECULAR ECOLOGY NOTES
影响因子：
--
作者：
Agapow, PM;Burt, A
通讯作者：
Burt, A
影响因子：
6.6
作者：
Schliep, Klaus;Potts, Alastair J.;Grimm, Guido W.
通讯作者：
Grimm, Guido W.