HectoSTAR μLED Optoelectrodes for Large-Scale, High-Precision In Vivo Opto-Electrophysiology.
HectoSTAR μLED Optoelectrodes for Large-Scale, High-Precision In Vivo Opto-Electrophysiology.
复制标题
DOI：
10.1002/advs.202105414
复制
发表时间：
2022-06
期刊：
影响因子：
15.1
通讯作者：
Yoon, Euisik
中科院分区：
文献类型：
--
作者：
Voroslakos, Mihaly;Kim, Kanghwan;Slager, Nathan;Ko, Eunah;Oh, Sungjin;Parizi, Saman S.;Hendrix, Blake;Seymour, John P.;Wise, Kensall D.;Buzsaki, Gyorgy;Fernandez-Ruiz, Antonio;Yoon, Euisik
大脑区域内部和跨区域的动态交互作用是行为和认知功能的基础。为了理解这些过程的基础，必须同步记录行为良好的动物大脑中分布的局部电路的活动，同时精确地操纵这些电路的输入。尽管最近的技术进步使这种大规模记录能力成为可能，但伴随这些记录的高时空分辨率和大规模调制技术的发展仍然滞后。在这项工作中，提出了一种新型的神经探针，它能够以高时空分辨率同时对深部神经元电路进行大范围的电学监测和光遗传操作。HETTOSTAR微发光二极管(μLED)光电电极采用256个记录电极和128个刺激μLED，单片集成在其四个30微米厚的硅微针柄的表面，覆盖了大体积，1.3mx0.9 mm的横截面面积位于大脑内部深达600万mm。该设备的使用在行为小鼠解剖跨皮层和海马区的长距离网络互动被证明。μ发光二极管光电电极的记录和刺激能力将为基于细胞和电路的行为动物大脑功能研究开辟新的可能性。提出了一种新型的神经探针，它可以同时进行大范围的电生理记录和光遗传操作，具有很高的时空分辨率。HettoSTAR微光发射二极管(μLED)光电极具有256个记录电极和128个密集集成在四个微针尖端的刺激性μLED，允许对行为动物进行基于细胞和电路的脑图绘制。
Dynamic interactions within and across brain areas underlie behavioral and cognitive functions. To understand the basis of these processes, the activities of distributed local circuits inside the brain of a behaving animal must be synchronously recorded while the inputs to these circuits are precisely manipulated. Even though recent technological advances have enabled such large‐scale recording capabilities, the development of the high‐spatiotemporal‐resolution and large‐scale modulation techniques to accompany those recordings has lagged. A novel neural probe is presented in this work that enables simultaneous electrical monitoring and optogenetic manipulation of deep neuronal circuits at large scales with a high spatiotemporal resolution. The “hectoSTAR” micro‐light‐emitting‐diode (μLED) optoelectrode features 256 recording electrodes and 128 stimulation μLEDs monolithically integrated on the surface of its four 30‐µm thick silicon micro‐needle shanks, covering a large volume with 1.3‐mm × 0.9‐mm cross‐sectional area located as deep as 6 mm inside the brain. The use of this device in behaving mice for dissecting long‐distance network interactions across cortical layers and hippocampal regions is demonstrated. The recording‐and‐stimulation capabilities hectoSTAR μLED optoelectrodes enables will open up new possibilities for the cellular and circuit‐based investigation of brain functions in behaving animals. A novel neural probe that enables simultaneous electrophysiological recording and optogenetic manipulation of deep neuronal circuits at large scales with a high spatiotemporal resolution is presented. The hectoSTAR micro‐light‐emitting‐diode (μLED) optoelectrode, featuring 256 recording electrodes and 128 stimulation μLEDs densely integrated on the tip of four micro‐needles, allows for cellular and circuit‐based brain mapping in behaving animals.
登录
查看更多内容
影响因子：
25
作者：
通讯作者：
--
DOI：
10.1126/science.abf3119
发表时间：
2021-04-02
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Fernández-Ruiz A;Oliva A;Soula M;Rocha-Almeida F;Nagy GA;Martin-Vazquez G;Buzsáki G
通讯作者：
Buzsáki G
影响因子：
3.8
作者：
ARRIDGE, SR;SCHWEIGER, M;DELPY, DT
通讯作者：
DELPY, DT
影响因子：
2.5
作者：
Csicsvari, J;Henze, DA;Buzsáki, G
通讯作者：
Buzsáki, G
影响因子：
25
作者：
Boyden, ES;Zhang, F;Deisseroth, K
通讯作者：
Deisseroth, K