Accelerated cell sheet detachment by copolymerizing hydrophilic PEG side chains into PNIPAm nanocomposite hydrogels
Accelerated cell sheet detachment by copolymerizing hydrophilic PEG side chains into PNIPAm nanocomposite hydrogels
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通过将亲水性 PEG 侧链共聚到 PNIPAm 纳米复合水凝胶中加速细胞片脱离
DOI：
10.1088/1748-6041/7/5/055008
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发表时间：
2012-09
影响因子：
4
通讯作者：
D. Liu, T. Wang, X. Liu, Z. Tong
中科院分区：
文献类型：
--
作者：
D. Liu, T. Wang, X. Liu, Z. Tong
以锂皂石粘土Laponite XLS为水悬浮液，通过原位聚合法将聚（N-异丙基丙烯酰胺）（PNIPAm）单体和聚乙二醇（PEG）大分子单体引入到PNIPAm纳米复合水凝胶（NC凝胶）中。用傅里叶变换红外光谱和X射线光电子能谱表征了NC凝胶的组成，用DSC和透射率表征了相分离温度，用动态力学谱和溶胀比表征了凝胶的相互作用。增加PEG含量导致共聚NC凝胶的储能模量和下临界溶解温度（LCST）略有增加，共聚NC凝胶的LCST仍低于37 °C。这些NC凝胶表面上的L929细胞粘附和增殖不受亲水性PEG侧链掺入的抑制。通过将温度降低到低于LCST，细胞片自发地从共聚的NC凝胶分离。用原子力显微镜和接触角测定仪对NC凝胶的表面形貌和表面润湿性进行了表征。发现由少量PEG侧链诱导的粗糙且亲水的表面有利于加速细胞片脱离，这可能是由于增强了水渗透到凝胶-细胞片界面中。
One-end-connected short poly(ethylene glycol) (PEG) side chains were facilely introduced into the poly(N-isopropylacrylamide) (PNIPAm) nanocomposite hydrogel (NC gel) via in situ copolymerization of NIPAm monomer and PEG macromonomer in the aqueous suspension of hectorite clay Laponite XLS. The NC gels were characterized with Fourier transform infrared and x-ray photoelectron spectroscopy for the composition, DSC and transmittance for the phase separation temperature, dynamic mechanical spectra and swelling ratio for the interaction. Increasing the PEG content led to a small increase in the storage modulus and the lower critical solution temperature (LCST) of the copolymerized NC gels, and the LCST of the copolymerized NC gels was still below 37 °C. The L929 cell adhesion and proliferation on the surface of these NC gels were not suppressed by the incorporation of hydrophilic PEG side chains. By lowering temperature below the LCST, the cell sheet spontaneously detached from the copolymerized NC gels. The surface morphology and surface wettability of the NC gels were detected by atom force microscope and contact angle measurement. A rough and hydrophilic surface induced by a small amount of PEG side chains was found to be favorable to accelerate the cell sheet detachment, probably due to the enhanced water permeation into the gel–cell sheet interface.
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影响因子：
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作者：
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DOI：
10.1002/(sici)1097-4636(200004)50:1
发表时间：
2000-04
期刊：
Journal of biomedical materials research
影响因子：
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作者：
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通讯作者：
P. Sepulveda;Jon Binner;S. Rogero;O. Higa;J. C. Bressiani