Documenting the Sporangium Development of the Polypodiales Fern Pteris multifida.
Documenting the Sporangium Development of the Polypodiales Fern Pteris multifida.
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记录水龙骨蕨 Pteris multifidi 的孢子囊发育
DOI：
10.3389/fpls.2022.878693
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
重建无种子维管植物中孢子囊的发育提供了有关关键过程的关键信息，这些过程在这些植物的生命周期中非常重要。本研究通过应用染料荧光成像技术和共聚焦显微镜技术对完整组织进行成像，旨在重建孢子囊发育过程中的关键步骤。特别强调了绒毡层和孢子母细胞的细胞壁结构，这些结构在过去受到显微镜文献的挑战。用荧光钙白色和碱性品红对多裂凤尾蕨孢子囊的细胞壁和细胞质进行染色后，在共聚焦显微镜下观察孢子囊的发育过程。共聚焦显微镜显示了从孢子囊起始细胞到柄、表皮、内绒毡层、外绒毡层和造孢细胞的清晰细胞谱系。本文对蕨类植物孢子囊发育的研究有助于对蕨类植物孢子形成的全面认识。
Reconstructing the development of sporangia in seed-free vascular plants provides crucial information about key processes enabling the production of spores that are important in the life cycle of these plants. By applying fluorescence imaging in intact tissues using dyes and confocal microscopy, this study aimed to reconstruct the key steps during the development of sporangia. Special emphasis was taken on the cell wall structures of tapetum and spore mother cells that have been challenged by microscopical documentation in the past. After staining the cell wall and cytoplasm using calcofluor white and basic fuchsin, the sporangium development of Pteris multifida was observed using confocal microscopy. The clear cell lineages from the sporangial initial cell to stalk, epidermis, inner tapetum, outer tapetum, and sporogenous cells were revealed by confocal imaging. The sporangium development improved in this work will be useful for a general understanding of fern spore formation.
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DOI：
10.1242/dev.127613
发表时间：
2015-12-01
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Kurihara D;Mizuta Y;Sato Y;Higashiyama T
通讯作者：
Higashiyama T
影响因子：
56.9
作者：
Noblin, X.;Rojas, N. O.;Dumais, J.
通讯作者：
Dumais, J.
影响因子：
3.4
作者：
Long, Hua;Li, Jing;Li, Li
通讯作者：
Li, Li
影响因子：
0.8
作者：
Wilson, KA
通讯作者：
Wilson, KA
影响因子：
--
作者：
Sanders, PM;Bui, AQ;Goldberg, RB
通讯作者：
Goldberg, RB