Visualization of microscale particle focusing in diluted and whole blood using particle trajectory analysis.
Visualization of microscale particle focusing in diluted and whole blood using particle trajectory analysis.
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DOI：
10.1039/c2lc21100a
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发表时间：
2012-06-21
期刊：
影响因子：
6.1
通讯作者：
Toner M
中科院分区：
文献类型：
--
作者：
Lim EJ;Ober TJ;Edd JF;McKinley GH;Toner M
惯性微流体技术已经证明了提供丰富的能力来操纵生物流体和颗粒以解决生物医学科学和临床医学中的各种挑战的潜力。各种微通道几何形状已被用于研究悬浮在简单缓冲溶液或高度稀释的血液中的颗粒的惯性聚焦行为。惯性聚焦的一个方面尚未研究的是悬浮在全血或最低稀释血液中的颗粒如何响应微通道中的惯性力。成像技术的效用（即，高速明场成像和长曝光荧光（条纹）成像）主要用于观察微通道中的颗粒聚焦，但由于大量红细胞（RBC）的干扰，在复杂流体如全血中受到限制。在这项研究中，我们使用粒子轨迹分析（PTA）观察聚苯乙烯珠，白色血细胞和PC-3前列腺癌细胞在生理盐水和血液中的惯性聚焦行为。在平均粒子速度高达1.85 m s−1时，实现了对聚焦（荧光标记）粒子的识别。使用聚焦颗粒的定量测量来构建通道横截面中颗粒频率的强度图和颗粒质心坐标与颗粒直径的散点图。加入全血（HCT = 45%）的PC-3细胞显示了在生理盐水或稀释血液中未观察到的新聚焦模式。PTA可用作实验参考系，用于理解全血中惯性升力的物理基础，并发现可用于实现全血中颗粒分离的惯性聚焦模式。
Inertial microfluidics has demonstrated the potential to provide a rich range of capabilities to manipulate biological fluids and particles to address various challenges in biomedical science and clinical medicine. Various microchannel geometries have been used to study the inertial focusing behavior of particles suspended in simple buffer solutions or in highly diluted blood. One aspect of inertial focusing that has not been studied is how particles suspended in whole or minimally diluted blood respond to inertial forces in microchannels. The utility of imaging techniques (i.e., high-speed bright-field imaging and long exposure fluorescence (streak) imaging) primarily used to observe particle focusing in microchannels is limited in complex fluids such as whole blood due to interference from the large numbers of red blood cells (RBCs). In this study, we used particle trajectory analysis (PTA) to observe the inertial focusing behavior of polystyrene beads, white blood cells, and PC-3 prostate cancer cells in physiological saline and blood. Identification of in-focus (fluorescently labeled) particles was achieved at mean particle velocities of up to 1.85 m s−1. Quantitative measurements of in-focus particles were used to construct intensity maps of particle frequency in the channel cross-section and scatter plots of particle centroid coordinates vs. particle diameter. PC-3 cells spiked into whole blood (HCT = 45%) demonstrated a novel focusing mode not observed in physiological saline or diluted blood. PTA can be used as an experimental frame of reference for understanding the physical basis of inertial lift forces in whole blood and discover inertial focusing modes that can be used to enable particle separation in whole blood.
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DOI：
10.1073/pnas.0404036101
发表时间：
2004-07-20
影响因子：
11.1
作者：
Krivacic, RT;Ladanyi, A;Bruce, RH
通讯作者：
Bruce, RH
影响因子：
9.9
作者：
GOLDSMITH, HL;MARLOW, JC
通讯作者：
MARLOW, JC
DOI：
10.1088/0960-1317/18/6/065015
发表时间：
2008-06-01
影响因子：
2.3
作者：
Kim, Young Won;Yoo, Jung Yul
通讯作者：
Yoo, Jung Yul
影响因子：
2.4
作者：
Klein, S. A.;Posner, J. D.
通讯作者：
Posner, J. D.
DOI：
10.1073/pnas.0402937101
发表时间：
2004-07-06
影响因子：
11.1
作者：
Long, DS;Smith, ML;Damiano, ER
通讯作者：
Damiano, ER