Characterization of spatial and temporal expression pattern of SCG10 during zebrafish development.
Characterization of spatial and temporal expression pattern of SCG10 during zebrafish development.
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DOI：
10.1016/j.gep.2008.12.010
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发表时间：
2009-04
期刊：
影响因子：
--
通讯作者：
Shepherd I
中科院分区：
文献类型：
--
作者：
Burzynski GM;Delalande JM;Shepherd I
SCG 10（上级颈神经节10，STMN 2）是stathmin蛋白家族的成员。Stathmins通过抑制聚合和促进解聚来调节微管动力学。SCG 10被认为是神经元特异性stathmin，其在发育神经元的生长锥中富集，并在调节神经突生长中起作用。在迄今为止检查的所有物种中，SCG 10在CNS和PNS中都表达。我们克隆了两个斑马鱼SCG 10同源基因，并通过RT-PCR和原位杂交确定了这两个基因的时空表达模式。RT-PCR结果表明，这两个转录本表达的母系和合子通过至少5天。原位杂交分析表明，这两个SCG 10直系同源物具有动态的，空间的表达模式，几乎是彼此相同的。最初，这些直系同源物在前脑、中脑和后脑的离散区域以及前外侧线神经节和后外侧线神经节中表达，并在脊髓Rohon-Beard神经元中短暂表达。从48 hpf开始，两种基因的表达水平增加，并主要限于前CNS（前脑区，视网膜，视顶盖和后脑）和颅神经节。从72到96 hpf，SCG 10基因也在肠和周围肠间充质中的发育神经元中表达。我们的研究结果提供了一个起点，为未来的研究，将调查在斑马鱼神经发育的SCG 10直系同源物的体内功能。
SCG10 (Superior Cervical Ganglia 10, STMN2) is a member of the stathmin family of proteins. Stathmins regulate microtubule dynamics by inhibiting polymerization and promoting their depolymerization. SCG10 is believed to be a neuronal-specific stathmin that is enriched in the growth cones of developing neurons and plays a role in regulating neurite outgrowth. In all species examined so far, SCG10 is expressed in both the CNS and PNS. We have cloned two zebrafish SCG10 homologues and have determined the temporal and spatial expression pattern of both of these genes by RT-PCR and in situ hybridization. RT-PCR shows that both transcripts are expressed maternally and zygotically through at least 5 days. In situ hybridization analysis reveals that both SCG10 orthologues have dynamic, spatial expression patterns that are nearly identical to each other. Initially, these orthologues are expressed in discrete areas of the forebrain, midbrain, and hindbrain, as well as in the anterior and posterior lateral line ganglia and transiently in the spinal cord Rohon-Beard neurons. From 48hpf onwards, the level of expression of both genes increases and becomes mainly restricted to the anterior CNS (the forebrain region, retina, optic tectum and hindbrain), and to the cranial ganglia. From 72 to 96 hpf, SCG10 genes are also expressed in the developing neurons in the gut and in the surrounding intestinal mesenchyme. Our results provide a starting point for future studies that will investigate the in vivo function of SCG10 orthologues in zebrafish neural development.
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DOI：
10.1002/neu.20295
发表时间：
2006-09-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
Morii, Hiroshi;Shiraishi-Yamaguchi, Yoko;Mori, Nozomu
通讯作者：
Mori, Nozomu
影响因子：
2.5
作者：
KIMMEL, CB;BALLARD, WW;SCHILLING, TF
通讯作者：
SCHILLING, TF
影响因子：
2.9
作者：
Manna, Tapas;Grenningloh, Gabriele;Wilson, Leslie
通讯作者：
Wilson, Leslie
影响因子：
4.8
作者：
Nakao, C;Itoh, TJ;Mori, N
通讯作者：
Mori, N
影响因子：
2.5
作者：
An, M;Luo, RS;Henion, PD
通讯作者：
Henion, PD