Dependence of exhaled breath composition on exogenous factors, smoking habits and exposure to air pollutants.
Dependence of exhaled breath composition on exogenous factors, smoking habits and exposure to air pollutants.
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DOI：
10.1088/1752-7155/6/3/036008
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发表时间：
2012-09
影响因子：
3.8
通讯作者：
Amann A
中科院分区：
文献类型：
--
作者：
Filipiak W;Ruzsanyi V;Mochalski P;Filipiak A;Bajtarevic A;Ager C;Denz H;Hilbe W;Jamnig H;Hackl M;Dzien A;Amann A
基于挥发性呼吸标记物的非侵入性疾病监测是一项非常有吸引力但具有挑战性的任务。使用现代分析技术（如质子转移反应质谱法、气相色谱-质谱法）在呼出的空气中检测到数百种化合物，这些化合物甚至与各种疾病有关。然而，在呼吸样本中检测到的大多数化合物的生化背景尚未阐明；因此，获得的结果应小心解释，以避免虚假的相关性。这项研究的主要目的是评估吸烟对呼出气体成分的影响。此外，在借鉴前人研究的基础上，重点从饮食、环境暴露和生物途径等方面探讨了呼吸中挥发性有机化合物（VOCs）的潜在来源。本文介绍了来自50名志愿者的115名受试者的呼气和吸入空气样本中检测到的挥发性有机化合物的概况。呼气样品采用多床吸附管预富集后的气相色谱-质谱分析，尿液样品采用固相微萃取（SPME）分析。在至少10%的志愿者呼出的气体中总共发现了266种化合物。通过光谱库匹配和保留时间（基于参考标准）鉴定出162个化合物。研究表明，暴露于污染和室内空气污染物，特别是吸烟，对呼出气体的成分有很大影响。超过80种有机化合物被发现与吸烟显著相关，其中最大的一类是不饱和碳氢化合物（29种二烯，27种烯烃和3种炔烃）。根据目前的结果，我们建议，为了将来对呼吸数据的理解，有必要仔细研究挥发物的潜在生物学来源，例如，通过分析组织、分离细胞系或其他体液。特别是，在临床诊断时应谨慎考虑与吸烟习惯有关或人类接触的挥发性有机化合物，因为其浓度谱的微小变化（通常在pptv-ppbv范围内）表明，某些疾病的爆发可能受到已经很高的本底的阻碍。
Non-invasive disease monitoring on the basis of volatile breath markers is a very attractive but challenging task. Several hundreds of compounds have been detected in exhaled air using modern analytical techniques (e.g. proton-transfer reaction mass spectrometry, gas chromatography-mass spectrometry) and have even been linked to various diseases. However, the biochemical background for most of compounds detected in breath samples has not been elucidated; therefore, the obtained results should be interpreted with care to avoid false correlations. The major aim of this study was to assess the effects of smoking on the composition of exhaled breath. Additionally, the potential origin of breath volatile organic compounds (VOCs) is discussed focusing on diet, environmental exposure and biological pathways based on other’s studies. Profiles of VOCs detected in exhaled breath and inspired air samples of 115 subjects with addition of urine headspace derived from 50 volunteers are presented. Samples were analyzed with GC-MS after preconcentration on multibed sorption tubes in case of breath samples and solid phase micro-extraction (SPME) in the case of urine samples. Altogether 266 compounds were found in exhaled breath of at least 10% of the volunteers. From these, 162 compounds were identified by spectral library match and retention time (based on reference standards). It is shown that the composition of exhaled breath is considerably influenced by exposure to pollution and indoor-air contaminants and particularly by smoking. More than 80 organic compounds were found to be significantly related to smoking, the largest group comprising unsaturated hydrocarbons (29 dienes, 27 alkenes and 3 alkynes). On the basis of the presented results, we suggest that for the future understanding of breath data it will be necessary to carefully investigate the potential biological origin of volatiles, e.g., by means of analysis of tissues, isolated cell lines or other body fluids. In particular, VOCs linked to smoking habit or being the results of human exposure should be considered with care for clinical diagnosis since small changes in their concentration profiles (typically in the pptv–ppbv range) revealing that the outbreak of certain disease might be hampered by already high background.
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影响因子：
3.8
作者：
Fisher, JW;Dorman, DC;Conolly, RB
通讯作者：
Conolly, RB
影响因子：
1.8
作者：
Abbott, SM;Elder, JB;Smith, D
通讯作者：
Smith, D
DOI：
10.1007/s00420-007-0233-9
发表时间：
2008-02-01
影响因子：
3
作者：
Janasik, Beata;Jakubowski, Marek;Jalowiecki, Piotr
通讯作者：
Jalowiecki, Piotr
影响因子：
2.1
作者：
Amárita, F;Fernández-Esplá, D;Pelaez, C
通讯作者：
Pelaez, C
影响因子：
3.8
作者：
Erhart, S.;Amann, A.;Scholl-Buergi, S.
通讯作者：
Scholl-Buergi, S.