Oriented Nanofibrous Polymer Scaffolds Containing Protein-Loaded Porous Silicon Generated by Spray Nebulization.
Oriented Nanofibrous Polymer Scaffolds Containing Protein-Loaded Porous Silicon Generated by Spray Nebulization.
复制标题
喷雾雾化产生的含有蛋白质的多孔硅的定向纳米纤维聚合物支架。
DOI：
10.1002/adma.201706785
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发表时间：
2018-03
期刊：
影响因子：
--
通讯作者：
Sailor MJ
中科院分区：
文献类型：
--
作者：
Zuidema JM;Kumeria T;Kim D;Kang J;Wang J;Hollett G;Zhang X;Roberts DS;Chan N;Dowling C;Blanco-Suarez E;Allen NJ;Tuszynski MH;Sailor MJ
通过使用喷枪从氯仿溶液喷雾雾化制备由嵌入聚己内酯（PCL）或聚（丙交酯-共-乙交酯）（PLGA）基质中的多孔硅纳米颗粒（pSiNPs）组成的取向复合纳米纤维。纳米纤维可以通过喷枪喷嘴的适当定位来定向，并且它们可以指导大鼠背根神经节神经元的神经突起的生长。当负载有模型蛋白溶菌酶时，pSiNP允许产生在生理条件（PBS，37°C）下携带和递送蛋白质长达60天的生物相容性支架，在该时间段内保留75%的酶活性。pSiNP中蛋白质的质量负载为36%，并且在所得聚合物/pSiNP支架中为2.5%。使用显示固有光致发光（来自量子限制的Si纳米结构）的pSiNP允许聚合物/pSiNP复合材料通过时间选通光致发光成像被明确地识别和跟踪。pSiNP在加工过程中和长期水性释放研究期间保护蛋白质有效载荷免于变性的显著能力建立了用于产生可生物降解的生物可降解支架的模型，该支架可以装载和递送敏感的生物制剂。使用喷枪制备混合蛋白质洗脱纳米纤维。蛋白质被隔离在多孔硅纳米颗粒中，其保留了蛋白质的活性并允许其从非水溶剂中沉积。纤维可以排列以指导细胞生长，并且它们能够释放活性蛋白超过60天。
Oriented composite nanofibers consisting of porous silicon nanoparticles (pSiNPs) embedded in a polycaprolactone (PCL) or poly(lactide-co-glycolide) (PLGA) matrix are prepared by spray nebulization from chloroform solutions using an airbrush. The nanofibers can be oriented by appropriate positioning of the airbrush nozzle, and they can direct growth of neurites from rat dorsal root ganglion neurons. When loaded with the model protein lysozyme, the pSiNPs allow the generation of nanofiber scaffolds that carry and deliver the protein under physiologic conditions (PBS, 37°C) for up to 60 days, retaining 75% of the enzymatic activity over this time period. The mass loading of protein in the pSiNPs is 36%, and in the resulting polymer/pSiNP scaffolds it is 2.5%. The use of pSiNPs that display intrinsic photoluminescence (from the quantum-confined Si nanostructure) allows the polymer/pSiNP composites to be definitively identified and tracked by time-gated photoluminescence imaging. The remarkable ability of the pSiNPs to protect the protein payload from denaturation, both during processing and for the duration of the long-term aqueous release study, establishes a model for the generation of biodegradable nanofiber scaffolds that can load and deliver sensitive biologics. Hybrid protein-eluting nanofibers are prepared using an airbrush. The protein is sequestered in a porous silicon nanoparticle, which retains the activity of the protein and allows its deposition from a non-aqueous solvent. The fibers can be aligned to direct cellular growth, and they are capable of releasing active protein for > 60 days.
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影响因子：
16.1
作者：
Capulli AK;MacQueen LA;Sheehy SP;Parker KK
通讯作者：
Parker KK
影响因子：
4.6
作者：
Gongalsky MB;Osminkina LA;Pereira A;Manankov AA;Fedorenko AA;Vasiliev AN;Solovyev VV;Kudryavtsev AA;Sentis M;Kabashin AV;Timoshenko VY
通讯作者：
Timoshenko VY
影响因子：
14
作者：
Determan, AS;Wilson, JH;Narasimhan, B
通讯作者：
Narasimhan, B
影响因子：
29.4
作者：
Canham, LT
通讯作者：
Canham, LT
DOI：
10.1002/pssa.200461134
发表时间：
2005-06-01
影响因子：
2
作者：
Coffer, JL;Whitehead, MA;Canham, LT
通讯作者：
Canham, LT