Anatomical Transcriptome Atlas of the Male Mouse Reproductive System During Aging.
Anatomical Transcriptome Atlas of the Male Mouse Reproductive System During Aging.
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DOI：
10.3389/fcell.2021.782824
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发表时间：
2021
影响因子：
5.5
通讯作者：
Xie Y
中科院分区：
文献类型：
--
作者：
Huang Y;Li X;Sun X;Yao J;Gao F;Wang Z;Hu J;Wang Z;Ouyang B;Tu X;Zou X;Liu W;Lu M;Deng C;Yang Q;Xie Y
老年男性的生育能力和睾丸内分泌功能退化，危及生殖健康和福祉。然而，生殖衰老的机制尚不清楚。在这里，我们试图解决这一问题，通过调查雄性小鼠生殖道的7个区域的表型和转录组：睾丸，输出小管，初始段，头，体和尾附睾，输精管，在成年（3个月）和老年（21个月）小鼠。采用定量PCR、免疫组化、免疫荧光染色和酶联免疫吸附试验对小鼠、人体组织和精液样本中的基因表达进行分析。老年雄性小鼠出现了系统和生殖系统的变化，睾丸和附睾近端出现了明显的组织学变化。绘制了雄性生殖道的转录组，并在小鼠和/或人组织中鉴定和验证了一系列区域特异性基因，包括鱼精蛋白1（Prm 2）、ADAM金属肽酶结构域28（Adam 28）、核糖核酸酶A家族成员13（Rnase 13）、WAP四-二硫键核心结构域13（Wfdc 13）和Wfdc 9。同时，男性生殖道不同区域的年龄相关的转录组变化的特点。值得注意的是，增加的免疫应答与男性生殖衰老，特别是T细胞活化功能有关。免疫反应相关因子磷脂酶A2 IID组（Pla 2g 2d）被确定为小鼠生殖衰老的潜在生物标志物。人精浆中的PLA 2G 2D水平在大约35岁时激增。此外，我们强调蛋白酪氨酸磷酸酶受体C型（Ptprc），淋巴细胞蛋白酪氨酸激酶（Lck），微管相关蛋白tau（Mapt），和干扰素诱导的蛋白与tetratricopeptide repeats 3（Ifit 3）的老化中的关键分子的初始段，头，头，尾附睾，分别。这项研究为小鼠衰老过程中的男性生殖系统提供了RNA-seq资源，有望提高我们对男性生殖衰老和不育的认识。
The elderly males undergo degenerative fertility and testicular endocrine function that jeopardize the reproductive health and well-being. However, the mechanisms underlying reproductive aging are unclear. Here, we tried to address this by investigating the phenotypes and transcriptomes of seven regions of the male mouse reproductive tract: the testis, efferent ductules, initial segment, caput, corpus and cauda epididymidis, and vas deferens, in adult (3 months) and aged (21 months) mice. Quantitative PCR, immunohistochemistry, immunofluorescent staining, and enzyme-linked immunosorbent assay were performed for the analysis of gene expression in mice, human tissues, and semen samples. Aged male mice showed both systematic and reproductive changes, and remarkable histological changes were detected in the testis and proximal epididymis. Transcriptomes of the male reproductive tract were mapped, and a series of region-specific genes were identified and validated in mouse and/or human tissues, including Protamine 1 (Prm2), ADAM metallopeptidase domain 28 (Adam28), Ribonuclease A family member 13 (Rnase13), WAP four-disulfide core domain 13 (Wfdc13), and Wfdc9. Meanwhile, age-related transcriptome changes of different regions of the male reproductive tract were characterized. Notably, increased immune response was functionally related to the male reproductive aging, especially the T cell activation. An immune response-associated factor, phospholipase A2 group IID (Pla2g2d), was identified as a potential biomarker for reproductive aging in mice. And the PLA2G2D level in human seminal plasma surged at approximately 35 years of age. Furthermore, we highlighted Protein tyrosine phosphatase receptor type C (Ptprc), Lymphocyte protein tyrosine kinase (Lck), Microtubule associated protein tau (Mapt), and Interferon induced protein with tetratricopeptide repeats 3 (Ifit3) as critical molecules in the aging of initial segment, caput, caput, and cauda epididymidis, respectively. This study provides an RNA-seq resource for the male reproductive system during aging in mice, and is expected to improve our understanding of male reproductive aging and infertility.
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影响因子：
64.8
作者：
Kong A;Frigge ML;Masson G;Besenbacher S;Sulem P;Magnusson G;Gudjonsson SA;Sigurdsson A;Jonasdottir A;Jonasdottir A;Wong WS;Sigurdsson G;Walters GB;Steinberg S;Helgason H;Thorleifsson G;Gudbjartsson DF;Helgason A;Magnusson OT;Thorsteinsdottir U;Stefansson K
通讯作者：
Stefansson K
影响因子：
11.8
作者：
Green CD;Ma Q;Manske GL;Shami AN;Zheng X;Marini S;Moritz L;Sultan C;Gurczynski SJ;Moore BB;Tallquist MD;Li JZ;Hammoud SS
通讯作者：
Hammoud SS
影响因子：
7.7
作者：
Li, Dianrong;Meng, Lingjun;Wang, Xiaodong
通讯作者：
Wang, Xiaodong
影响因子：
6.7
作者：
Haraguchi, Takahiro;Ishikawa, Tomomoto;Fujisawa, Masato
通讯作者：
Fujisawa, Masato
影响因子：
3.6
作者：
Jelinsky, Scott A.;Turner, Terry T.;Johnston, Daniel S.
通讯作者：
Johnston, Daniel S.