Whole genome duplications and expansion of the vertebrate GATA transcription factor gene family.
Whole genome duplications and expansion of the vertebrate GATA transcription factor gene family.
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DOI：
10.1186/1471-2148-9-207
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发表时间：
2009-08-20
影响因子：
3.4
通讯作者：
Schneider SQ
中科院分区：
文献类型：
--
作者：
Gillis WQ;St John J;Bowerman B;Schneider SQ
GATA转录因子影响许多发育过程，包括胚胎胚层的指定。GATA基因家族在许多动物中都有显著的扩展：虽然不同的线虫只有一个GATA转录因子，但在许多脊椎动物中发现了6个GATA基因，在许多昆虫中发现了5个基因，在线虫中发现了11到13个GATA基因。所有双边动物基因组都至少有两个类别中的一个成员，即GATA123和GATA456。我们已经从两种无脊椎动物后口动物的基因组序列中鉴定出一种GATA123基因和一种GATA456基因，一种头口动物(Branchiostoma Floridae)和一种半鱼目动物(Saccoglossus Kowalevskii)。我们还证实了在所有脊椎动物基因组中存在六个GATA基因，以及在硬骨鱼中存在其他GATA基因。对保守序列基序和外显子-内含子结构变化的分析以及这些后口GATA基因的分子系统发育分析支持它们来自两个祖先的后口基因，一个是GATA 123，一个是GATA456。对脊椎动物之间保守的基因组组织进行比较，发现了18个与多个脊椎动物GATA基因连锁的平行基因家族(GATA Paralogons)，这为脊椎动物GATA基因家族通过基因组复制事件扩大提供了迄今最有力的证据。从我们的分析中，我们推断了脊椎动物GATA转录因子之间的进化出生顺序和关系，并通过多轮全基因组复制事件来定义它们的扩展。由于四个独立的无脊椎动物后口脊椎动物的基因组包含单拷贝GATA123和GATA456基因，我们推测OR(前基因组复制)无脊椎动物后肢动物祖先也有两个GATA基因，每类一个。Synteny分析确定了平行染色体区域(Paralogons)的重复，从单个祖先脊椎动物的GATA123和GATA456染色体到第一轮脊椎动物基因组复制后的4个Paralogons，到第二轮脊椎动物基因组复制后的7个Paralogons，以及FISH特异性3R基因组复制后的14个Paralogons。对GATA基因起源和关系的进化分析可能有助于理解脊椎动物GATA因子的冗余和专门化。
GATA transcription factors influence many developmental processes, including the specification of embryonic germ layers. The GATA gene family has significantly expanded in many animal lineages: whereas diverse cnidarians have only one GATA transcription factor, six GATA genes have been identified in many vertebrates, five in many insects, and eleven to thirteen in Caenorhabditis nematodes. All bilaterian animal genomes have at least one member each of two classes, GATA123 and GATA456. We have identified one GATA123 gene and one GATA456 gene from the genomic sequence of two invertebrate deuterostomes, a cephalochordate (Branchiostoma floridae) and a hemichordate (Saccoglossus kowalevskii). We also have confirmed the presence of six GATA genes in all vertebrate genomes, as well as additional GATA genes in teleost fish. Analyses of conserved sequence motifs and of changes to the exon-intron structure, and molecular phylogenetic analyses of these deuterostome GATA genes support their origin from two ancestral deuterostome genes, one GATA 123 and one GATA456. Comparison of the conserved genomic organization across vertebrates identified eighteen paralogous gene families linked to multiple vertebrate GATA genes (GATA paralogons), providing the strongest evidence yet for expansion of vertebrate GATA gene families via genome duplication events. From our analysis, we infer the evolutionary birth order and relationships among vertebrate GATA transcription factors, and define their expansion via multiple rounds of whole genome duplication events. As the genomes of four independent invertebrate deuterostome lineages contain single copy GATA123 and GATA456 genes, we infer that the 0R (pre-genome duplication) invertebrate deuterostome ancestor also had two GATA genes, one of each class. Synteny analyses identify duplications of paralogous chromosomal regions (paralogons), from single ancestral vertebrate GATA123 and GATA456 chromosomes to four paralogons after the first round of vertebrate genome duplication, to seven paralogons after the second round of vertebrate genome duplication, and to fourteen paralogons after the fish-specific 3R genome duplication. The evolutionary analysis of GATA gene origins and relationships may inform understanding vertebrate GATA factor redundancies and specializations.
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影响因子：
3.9
作者：
Lowry, JA;Atchley, WR
通讯作者：
Atchley, WR
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作者：
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作者：
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通讯作者：
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