Temporal and Spatial Variation in the Abundance of Total and Pathogenic Vibrio parahaemolyticus in Shellfish in China.
Temporal and Spatial Variation in the Abundance of Total and Pathogenic Vibrio parahaemolyticus in Shellfish in China.
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DOI：
10.1371/journal.pone.0130302
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Chen Y
中科院分区：
文献类型：
--
作者：
Han H;Li F;Yan W;Guo Y;Li N;Liu X;Zhu J;Xu J;Chen Y;Li X;Lv H;Zhang Y;Cai T;Chen Y
采用最大可能数-聚合酶链反应（MPN-PCR）方法，对2013年5月至2014年3月采集的中国4个省市贝类样品中副溶血性弧菌的丰度进行了调查。总副溶血性弧菌检出率为67.7%。分别有38.1%和10.1%的样品超过1，000 MPN g-1和10，000 MPN g-1。副溶血性弧菌的密度随季节和地理分布而变化，广西和四川贝类的副溶血性弧菌总含量比辽宁和山东地区高100倍。此外，夏季和秋季的副溶血性弧菌水平至少比凉爽季节高10倍。致病性副溶血性弧菌水平通常比总副溶血性弧菌水平低几个对数单位，并且在被高总副溶血性弧菌水平污染的样本中往往较高。水产养殖场有一个较低的患病率，但较高的丰度总副溶血性弧菌相比，零售市场。餐饮市场的副溶血性弧菌总含量最低，但20.0%的样本超过1，000 MPN g-1。牡蛎中的总副溶血性弧菌和致病性副溶血性弧菌的水平均高于蛤。对数转换的副溶血性弧菌丰度与水温和气温均显著相关，但与海水盐度无关。这些结果提供了中国贝类中副溶血性弧菌污染的基线数据，可用于当地风险评估，以优先考虑重点部门的风险控制，并评估未来控制措施的有效性。
We investigated the abundance of total and pathogenic Vibrio parahaemolyticus in shellfish sampled from four provinces in China during May 2013 and March 2014 using the most probable number-polymerase chain reaction (MPN-PCR) method. Total V. parahaemolyticus was detected in 67.7% of 496 samples. A total of 38.1% and 10.1% of samples exceeded 1,000 MPN g-1 and 10,000 MPN g-1, respectively. V. parahaemolyticus densities followed a seasonal and geographical trend, with Guangxi and Sichuan shellfish possessing total V. parahaemolyticus levels that were 100-fold higher than those of the Liaoning and Shandong regions. Moreover, the levels of V. parahaemolyticus were at least 10-fold higher in the summer and autumn than in the cooler seasons. Pathogenic V. parahaemolyticus levels were generally lower than total V. parahaemolyticus levels by several log units and tended to be high in samples contaminated with high total V. parahaemolyticus levels. The aqua farms had a lower prevalence but higher abundance of total V. parahaemolyticus compared to retail markets. The catering markets showed the lowest levels of total V. parahaemolyticus, but 20.0% of samples exceeded 1,000 MPN g-1. The levels of both total and pathogenic V. parahaemolyticus in oysters were higher than in clams. The log-transformed abundance of V. parahaemolyticus was significantly correlated with both water temperature and air temperature but not water salinity. These results provide baseline contamination data of V. parahaemolyticus in shellfish in China, which can be applied to local risk assessments to prioritize risk control to key sectors and evaluate the effectiveness of future control measures.
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影响因子：
2.2
作者：
Bej, AK;Patterson, DP;Kaysner, CA
通讯作者：
Kaysner, CA
DOI：
10.1016/j.ijfoodmicro.2003.10.004
发表时间：
2004-04-15
影响因子：
5.4
作者：
Lin, C;Yu, RC;Chou, CC
通讯作者：
Chou, CC
影响因子：
4.4
作者：
DePaola, A;Ulaszek, J;Gendel, SM
通讯作者：
Gendel, SM
影响因子：
4.4
作者：
Nordstrom, Jessica L.;Vickery, Michael C. L.;DePaola, Angelo
通讯作者：
DePaola, Angelo
影响因子：
4.4
作者：
Myers, ML;Panicker, G;Bej, AK
通讯作者：
Bej, AK