Accurate and easy method for systemin quantification and examining metabolic changes under different endogenous levels.
Accurate and easy method for systemin quantification and examining metabolic changes under different endogenous levels.
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DOI：
10.1186/s13007-018-0301-z
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发表时间：
2018
期刊：
影响因子：
5.1
通讯作者：
Flors V
中科院分区：
文献类型：
--
作者：
Pastor V;Sánchez-Bel P;Gamir J;Pozo MJ;Flors V
系统素自发现以来一直被广泛研究，并被描述为番茄植物和其他茄科植物中的肽激素。茉莉酸和系统素与茉莉酸通过正反馈回路起作用，在响应创伤和昆虫攻击中发挥协同作用。尽管其生物学相关性，但大多数关于系统素在防御中的功能的研究都是通过前系统素（PROSYSTEMIN）基因表达进行研究的，该基因编码前肽前系统素，该前肽随后被切割为系统素（SYSTEMIN）。有趣的是，几乎没有任何研究是基于肽的定量。在这项研究中，一个简单而准确的定量方法系统素的开发，以了解其对植物代谢的影响。发现系统素的基础水平极低。为了研究内源性系统素对植物代谢的作用，在过表达PROTEINS基因的转基因株系（PS+）和沉默的反义株系（PS-）中对系统素进行了定量。我们通过非靶向代谢组学分析，通过与野生型植物相比，分析两个品系的代谢组学谱，评估了系统素在植物代谢中的相关性。与野生型植物和PS−系植物相比，PS+中木脂素生物合成和酪氨酸代谢途径内的化合物强烈积累。外源处理增强木脂素的积累，这证实了木脂素在细胞壁强化中的作用。出乎意料的是，PS+植物表现出野生型水平的茉莉酸（JA），但12-氧代-植物二烯酸（OPDA）的积累升高，这表明PS+不应该被用作JA的过度积累在实验设置。一个简单的方法，需要显着很少的样品操作，以量化的肽类化合物，描述。以前的研究是基于基因变化。在我们的研究中，在野生型番茄叶片中，番茄红素以极低的水平积累，在PROGREMIN过表达植物中显示出略高的水平，并且在沉默株系中不存在。这些微小的变化对植物的新陈代谢有重大影响。SA和OPDA，但不是JA，在PROSYS过表达植物中较高。本文的在线版本（10.1186/s13007-018-0301-z）包含补充材料，可供授权用户使用。
Systemin has been extensively studied since it was discovered and is described as a peptidic hormone in tomato plants and other Solanaceae. Jasmonic acid and systemin are proposed to act through a positive feed-back loop with jasmonic acid, playing synergistic roles in response to both wounding and insect attack. Despite its biological relevance, most studies regarding the function of systemin in defence have been studied via PROSYSTEMIN (PROSYS) gene expression, which encodes the propeptide prosystemin that is later cleaved to systemin (SYS). Interestingly, hardly any studies have been based on quantification of the peptide. In this study, a simple and accurate method for systemin quantification was developed to understand its impact on plant metabolism. The basal levels of systemin were found to be extremely low. To study the role of endogenous systemin on plant metabolism, systemin was quantified in a transgenic line overexpressing the PROSYS gene (PS+) and in a silenced antisense line (PS−). We evaluated the relevance of systemin in plant metabolism by analysing the metabolomic profiles of both lines compared to wildtype plants through untargeted metabolomic profiling. Compounds within the lignan biosynthesis and tyrosine metabolism pathways strongly accumulated in PS+ compared to wild-type plants and to plants from the PS− line. The exogenous treatments with SYS enhanced accumulation of lignans, which confirms the role of SYS in cell wall reinforcement. Unexpectedly, PS+ plants displayed wild-type levels of jasmonic acid (JA) but elevated accumulation of 12-oxo-phytodienoic acid (OPDA), suggesting that PS+ should not be used as an over-accumulator of JA in experimental setups. A simple method, requiring notably little sample manipulation to quantify the peptide SYS, is described. Previous studies were based on genetic changes. In our study, SYS accumulated at extremely low levels in wild-type tomato leaves, showed slightly higher levels in the PROSYSTEMIN-overexpressing plants and was absent in the silenced lines. These small changes have a significant impact on plant metabolism. SA and OPDA, but not JA, were higher in the PROSYS-overexpressing plants. The online version of this article (10.1186/s13007-018-0301-z) contains supplementary material, which is available to authorized users.
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影响因子：
3
作者：
Kaever A;Lingner T;Feussner K;Göbel C;Feussner I;Meinicke P
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