Low-temperature treatment of tobacco ovaries improves the isolation, viability, and embryogenic potential of two-celled proembryos
Low-temperature treatment of tobacco ovaries improves the isolation, viability, and embryogenic potential of two-celled proembryos
复制标题
烟草卵巢的低温处理提高了双细胞原胚的分离、活力和胚胎发生潜力
DOI：
10.1007/s11627-012-9447-y
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发表时间：
2012-07
期刊：
影响因子：
--
通讯作者：
Liang-Huan Qu
中科院分区：
文献类型：
--
作者：
Liang-Huan Qu
探讨了低温处理对烟草体外双细胞原胚分离及后续培养的影响。卵巢经4℃低温处理5 ~ 24 h后，两细胞前胚的分离效率显著提高，平均提高23.47%。冷处理后，两细胞前胚的细胞器分布、生长模式和细胞壁成分分布均保持良好的细胞活力和极性。此外，体外培养的两细胞原胚胎分裂正常，其时间与从新鲜卵巢分离的两细胞原胚胎平行。出乎意料的是，冷处理显著提高了两细胞原胚的分裂频率。以上结果表明，低温处理烟草子房有利于双细胞原胚的体外培养，不仅可以提高分离效率，还可以促进细胞分裂，提高进一步培养的潜力。
The influence of low-temperature treatment of ovaries on the isolation and subsequent culture of two-celled proembryoin vitrowas explored in tobacco. The efficiency of isolation of two-celled proembryos was significantly improved, by an average of 23.47 % after treatment of ovaries at 4°C for 5–24 h. Cell viability and polarity of two-celled proembryos, as assessed by organelle distribution, growth pattern, and cell wall component distribution, were all well maintained after cold treatment. Furthermore,in vitrocultivated two-celled proembryos divided normally, with timing paralleling that of two-celled proembryos isolated from fresh ovaries. Unexpectedly, the division frequency of two-celled proembryos was significantly improved by cold treatment. These results demonstrate that low-temperature treatment of tobacco ovaries was beneficial for two-celled proembryo manipulationin vitro, by not only improving isolation efficiency but also by promoting cell division and improving the potential for further cultivation.
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影响因子：
8.3
作者：
R. Malinowski;M. Filipecki
通讯作者：
R. Malinowski;M. Filipecki
影响因子：
9.5
作者：
Colette A. ten Hove;R. Heidstra
通讯作者：
Colette A. ten Hove;R. Heidstra
影响因子：
6.2
作者：
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通讯作者：
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影响因子：
2
作者：
Bolte, S;Talbot, C;Satiat-Jeunemaitre, B
通讯作者：
Satiat-Jeunemaitre, B
DOI：
--
发表时间：
2005
期刊：
--
影响因子：
--
作者：
R. Hadley;W. Hable;D. Kropf;Darryl L Edu;Kropf
通讯作者：
R. Hadley;W. Hable;D. Kropf;Darryl L Edu;Kropf