Induction of cytokine expression by leukemia inhibitory factor.
Induction of cytokine expression by leukemia inhibitory factor.
复制标题
白血病抑制因子诱导细胞因子表达。
DOI：
10.1172/jci116363
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Lotz,M
中科院分区：
文献类型：
--
作者：
Villiger,PM;Geng,Y;Lotz,M
细胞因子的生物学效应部分由它们在调节细胞因子产生中的相互作用决定。本研究分析了白血病抑制因子（LIF）对不同细胞系细胞因子表达的影响。重组人LIF增加人关节软骨细胞中IL-1 β、IL-6和IL-8 mRNA的水平，如通过北方印迹证明的。这些细胞因子mRNA早在刺激后1.3 h就可检测到，并在5 h后达到最大值。LIF的作用是剂量依赖性的，并且与IL-1的作用大小相似。通过代谢标记和免疫沉淀，表明LIF诱导IL-6的合成和分泌。通过B 9杂交瘤增殖测定法测量的条件培养基中的IL-6生物活性通过LIF增加。LIF对细胞因子表达的影响并不局限于结缔组织细胞。PCR结果显示，人血单核细胞经LIF刺激后表达IL-6 mRNA。刺激后2 h可检测到IL-6 mRNA水平增加，5 h开始下降。与IL-1 β相比，该反应的持续时间较短。除了增加mRNA表达外，LIF还刺激血液单核细胞释放生物活性IL-6。在滑膜细胞和神经元以及上皮细胞系中，LIF增加IL-1 β和IL-6基因表达。总之，LIF在多种组织中诱导细胞因子表达。这些结果表明，通过诱导细胞因子，LIF可以调节炎症，免疫反应和结缔组织代谢，并在不同的疾病状态中充当致病介质。图片
Biological effects of cytokines are in part determined by their interactions in the regulation of cytokine production. This study analyzes the effects of leukemia inhibitory factor (LIF) on cytokine expression in different cell lineages. Recombinant human LIF increases levels of IL-1 beta, IL-6, and IL-8 mRNA in human articular chondrocytes as demonstrated by Northern blotting. These cytokine mRNAs are detectable as early as 1.3 h after stimulation and reach their maximum after 5 h. The LIF effects are dose dependent and of similar magnitude to those of IL-1. By metabolic labeling and immunoprecipitation it is shown that LIF induces synthesis and secretion of IL-6. IL-6 bioactivity in conditioned media, as measured by the B9 hybridoma proliferation assay, is increased by LIF. Effects of LIF on cytokine expression are not confined to connective tissue cells. By PCR it is shown that human blood monocytes express IL-6 mRNA after stimulation with LIF. An increase in IL-6 mRNA levels is detectable 2 h after stimulation, and this starts to decline by 5 h. The response is of shorter duration as compared with IL-1 beta. In addition to increased mRNA expression, LIF also stimulates release of biologically active IL-6 from blood monocytes. In synoviocytes and neuronal as well as epithelial cell lines, LIF increases IL-1 beta and IL-6 gene expression. In summary, LIF induces cytokine expression in a wide variety of tissues. These results suggest that through the induction of cytokines, LIF can modulate inflammation, immune responses, and connective tissue metabolism, and act as a pathogenetic mediator in different disease states.Images
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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