Carboxyl-ebselen-based layer-by-layer films as potential antithrombotic and antimicrobial coatings.
Carboxyl-ebselen-based layer-by-layer films as potential antithrombotic and antimicrobial coatings.
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DOI：
10.1016/j.biomaterials.2011.06.075
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发表时间：
2011-11
期刊：
影响因子：
14
通讯作者：
Meyerhoff, Mark E.
中科院分区：
文献类型：
--
作者：
Cai, Wenyi;Wu, Jianfeng;Xi, Chuanwu;Ashe, Arthur J., III;Meyerhoff, Mark E.
通过将羧基-依布硒啉固定化的聚乙烯亚胺（e-PEI）和海藻酸钠（Alg）交替组装到基底上，然后通过盐退火和交联制备羧基-依布硒啉层层（LbL）膜。退火膜表现出显着改善的稳定性是能够产生一氧化氮（NO）的内源性S-亚硝基硫醇（RSNO）在还原剂的存在下。比较了不同有机硒物种在溶液相中的NO生成行为，并详细研究了形成稳定LbL膜的退火机制。LbL膜涂覆的聚氨酯导管即使在血液浸泡24小时后也能够从RSNO产生生理水平的NO，表明涂层的潜在抗血栓形成应用。此外，LbL膜还被证明能够减少活细菌表面附着并杀死广谱细菌，可能是通过从氧气产生超氧化物（O2·−）。这种类型的膜预期具有作为用于不同生物医学装置表面的抗血栓形成和抗微生物涂层的潜在应用。
A carboxyl-ebselen-based layer-by-layer (LbL) film was fabricated by alternatively assembling carboxyl-ebselen immobilized polyethylenimine (e-PEI) and alginate (Alg) onto substrates followed by salt annealing and cross-linking. The annealed films exhibiting significantly improved stability are capable of generating nitric oxide (NO) from endogeneous S-nitrosothiols (RSNOs) in the presence of a reducing agent. The NO generation behaviors of different organoselenium species in solution phase are compared and the annealing mechanism to create stable LbL films is studied in details. An LbL film coated polyurethane catheter is capable of generating physiological levels of NO from RSNOs even after blood soaking for 24 h, indicating potential antithrombotic applications of the coating. Further, the LbL film is also demonstrated to be capable of reducing living bacterial surface attachment and killing a broad spectrum of bacteria, likely through generation of superoxide (O2•−) from oxygen. This type of film is expected to have potential application as an antithrombotic and antimicrobial coating for different biomedical device surfaces.
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影响因子：
14
作者：
Riccio, Daniel A.;Dobmeier, Kevin P.;Hetrick, Evan M.;Privett, Benjamin J.;Paul, Heather S.;Schoenfisch, Mark H.
通讯作者：
Schoenfisch, Mark H.
影响因子：
14
作者：
Major, Terry C.;Brant, David O.;Reynolds, Melissa M.;Bartlett, Robert H.;Meyerhoff, Mark E.;Handa, Hitesh;Annich, Gail M.
通讯作者：
Annich, Gail M.
影响因子：
3.9
作者：
McAloney, RA;Dudnik, V;Goh, MC
通讯作者：
Goh, MC
影响因子：
17.1
作者：
Hetrick, Evan M.;Shin, Jae Ho;Schoenfisch, Mark H.
通讯作者：
Schoenfisch, Mark H.
影响因子：
5.2
作者：
van der Starre, Willize E.;van Nieuwkoop, Cees;van Dissel, Jaap T.
通讯作者：
van Dissel, Jaap T.