Study on Specific Apis cerana Honeybee Queen Pheromone Biosensor Based on Pheromone-Binding Protein ASP1
Study on Specific Apis cerana Honeybee Queen Pheromone Biosensor Based on Pheromone-Binding Protein ASP1
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基于信息素结合蛋白ASP1的中华蜜蜂蜂王信息素特异性生物传感器研究
DOI：
10.1109/jsen.2021.3054632
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发表时间：
2021-04
影响因子：
4.3
通讯作者：
Li Hong-Liang
中科院分区：
文献类型：
--
作者：
Zhang Jun-Fei;Wu Fan;Tang Ming-Zhu;Chen Dong-Xiao;Tan Jing;Liu Qing-Jun;Li Hong-Liang
生物传感器是一种由生物活性单元和信号转换器组成的检测装置。在一个群居的蜜蜂群体中，工蜂也进化出了一种复杂的嗅觉系统，专门感知蜂王信息素的成分。目前还不清楚是否有可能设计出一种利用工蜂嗅觉系统在体外检测蜂王费洛蒙的生物传感器。本研究利用硝酸纤维素膜将重组蜜蜂信息素结合蛋白1 （PBP1/ASP1）固定在叉形指金电极上，成功构建了一种特异的信息素生物传感器。结果表明，两种蜂后信息素成分[对羟基苯甲酸甲酯（HOB）和香草醇（HVA）]的最大电阻电荷转移（Rct）变化达到60%，显著高于蜂幼虫信息素成分（油酸乙酯）和花挥发物（$\ β $ -离子酮）。对HOB和HVA的线性检测范围为10−7 ~ 10−4 mol/L，理论检测限为10−8 mol/L，表明该生物传感器具有较宽的检测线性范围和较好的检测能力。这些结果表明，该生物传感器对蜂王信息素成分具有高度的敏感性和特异性。这有利于保护和维持未来蜜蜂资源的可持续性。
Biosensor is a detection device composed of a biologically active unit and a signal converter. In a eusocial honeybee colony, worker bees have also evolved a sophisticated olfactory system to specifically sense the queen pheromone components. So far, it is not clear whether it is possible to design a biosensor to detect the queen pheromone in vitro by using worker bees’ olfactory system. In this study, a specific pheromone biosensor was successfully constructed by immobilizing the recombinant honeybees’ pheromone binding protein 1 (PBP1/ASP1) on the forked finger gold electrode using nitrocellulose membrane. The results showed that the maximum resistance charge transfer (Rct) changes of two queen pheromone components [methyl p-hydroxy-benzoate (HOB) and vanillyl alcohol (HVA)] reached 60%, which were significantly higher than those of a bee larval pheromone component (ethyl oleate), and a floral volatile ( $\beta $ -ionone). The linear detection of HOB and HVA ranged from 10−7 to 10−4 mol/L, and the theoretical detection limit reached 10−8 mol/L, indicating the wide detection linear range and excellent detection capability of this biosensor. These results indicated that the biosensor was highly sensitive and specific to the queen pheromone components. It is beneficial to protect and maintain the sustainability of honeybee resources in the future.
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DOI：
10.1046/j.1432-1327.2001.01927.x
发表时间：
2001-02-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Briand, L;Nespoulous, C;Pernollet, JC
通讯作者：
Pernollet, JC
DOI：
10.1016/0305-0491(95)00019-5
发表时间：
1995-07-01
影响因子：
2.2
作者：
PELOSI, P;MAIDA, R
通讯作者：
MAIDA, R
基于蜜蜂化学感应蛋白的嗅觉生物传感器的阻抗传感和分子建模
影响因子：
12.6
作者：
Liu, Qingjun;Wang, Hua;Wang, Ping
通讯作者：
Wang, Ping
影响因子：
12.6
作者：
Ko, Hwi Jin;Park, Tai Hyun
通讯作者：
Park, Tai Hyun
DOI：
--
发表时间：
2000-11
期刊：
Journal of natural toxins
影响因子：
--
作者：
H. O. Lee;B. Cheun;J. Yoo;E. Watanabe;M. Han
通讯作者：
H. O. Lee;B. Cheun;J. Yoo;E. Watanabe;M. Han