Surface modification of the conducting polymer, polypyrrole, via affinity peptide.
Surface modification of the conducting polymer, polypyrrole, via affinity peptide.
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DOI：
10.1002/jbm.a.34435
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发表时间：
2013-05
影响因子：
4.9
通讯作者：
Schmidt, Christine E.
中科院分区：
文献类型：
--
作者：
Nickels, Jonathan D.;Schmidt, Christine E.
提出了一种基于亲和的导电聚合物聚吡咯（PPy）表面改性的新策略。先前通过噬菌体展示技术鉴定了一个12个氨基酸的肽（THRTSTLDYFVI，以下简称T59）。这种肽与氯掺杂的导电聚合物聚吡咯（PPyCl）非共价结合。先前的研究表明，导电聚合物在神经电极、传感器以及改善周围神经和其他组织的再生和愈合方面具有广阔的应用前景。因此，利用T59亲和肽将生物活性分子强而特异性地附着在PPy表面是一种令人兴奋的新方法，可以提高电活性材料的生物活性，用于各种生物医学应用。我们通过使用T59作为系链来修饰PPyCl与层粘连蛋白片段IKVAV以增强细胞相互作用，以及与所谓的隐形分子聚乙二醇（PEG）减少细胞相互作用来证明这一点。使用这两种修饰策略，我们能够控制PPy表面的细胞附着和神经突延伸，这对于不同的应用至关重要（即，组织再生的目标是增强细胞相互作用，而电极和传感器应用的目标是减少胶质细胞相互作用，从而减少瘢痕形成）。值得注意的是，PPyCl表面的导电性不受这种表面改性技术的影响，这与其他已经探索的表面改性导电聚合物的方法不同。最后，通过皮下植入，我们证实了经T59肽处理的PPyCl在体内的反应与未处理的PPyCl没有不同。
A novel strategy for affinity-based surface modification of the conducting polymer, polypyrrole, (PPy), has been developed. A 12-amino acid peptide (THRTSTLDYFVI, hereafter denoted T59) was previously identified via the phage display technique. This peptide non-covalently binds to the chlorine-doped conducting polymer polypyrrole (PPyCl). Studies have previously shown that conductive polymers have promising application in neural electrodes, sensors, and for improving regeneration and healing of peripheral nerves and other tissues. Thus, the strong and specific attachment of bio-active molecules to the surface of PPy using the T59 affinity peptide is an exciting new approach to enhance the bioactivity of electrically active materials for various biomedical applications. We demonstrate this by using T59 as a tether to modify PPyCl with the laminin fragment IKVAV to enhance cell interactions, as well as with the so-called stealth molecule poly(ethylene glycol; PEG) to decrease cell interactions. Using these two modification strategies, we were able to control cell attachment and neurite extension on the PPy surface, which is critical for different applications (i.e., the goal for tissue regeneration is to enhance cell interactions, whereas the goal for electrode and sensor applications is to reduce glial cell interactions and thus decrease scarring). Significantly, the conductivity of the PPyCl surface was unaffected by this surface modification technique, which is not the case with other methods that have been explored to surface modify conducting polymers. Finally, using subcutaneous implants, we confirmed that the PPyCl treated with the T59 peptide did not react in vivo differently than untreated PPyCl.
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