Genetic analysis of cold tolerance at the germination and booting stages in rice by association mapping.
Genetic analysis of cold tolerance at the germination and booting stages in rice by association mapping.
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水稻发芽和孕穗期耐寒性的关联图谱分析
DOI：
10.1371/journal.pone.0120590
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
Pan Y;Zhang H;Zhang D;Li J;Xiong H;Yu J;Li J;Rashid MA;Li G;Ma X;Cao G;Han L;Li Z
低温影响水稻生长的各个阶段。它可引起严重的幼苗伤害和雄性不育，导致严重的产量损失。利用174份中国水稻种质资源和273个SSR标记的小型核心种质，通过关联分析，研究了水稻萌发期和孕穗期的耐冷性及其遗传基础。籼粳亚种的两个不同居群在耐冷性及其遗传基础上存在明显差异。两个亚种在两个生长阶段对冷胁迫都很敏感。然而，粳稻比籼稻更宽容的所有阶段，衡量幼苗存活和结实。发芽期和孕穗期的耐冷性相关性较低。共检测到51个耐冷性数量性状基因座（QTL），分布在12条染色体上，其中发芽期检测到22个，孕穗期检测到33个。通过4个指标中的至少2个，共检测到8个QTL。约46%的QTL代表新位点。在相同的测量中，籼粳之间唯一共有的QTL是SSvR的qLTSSvR 6 -2。这暗示了两个亚种之间存在复杂的旧耐受机制。根据各基因型对各QTL的相对基因型效应（RGE），在籼稻中检测到18个阳性基因型和21个阴性基因型，在粳稻中检测到19个阳性基因型和24个阴性基因型。一般而言，在两个亚种中，负面影响远强于正面影响。在一个亚种中具有正效应的QTL标记被证明对该亚种的耐冷性选择是有效的，但在其他亚种中则不是。在分子标记辅助选择育种中，应尽量避免选择对耐冷性有强负效应的QTL，以免抵消其它位点上的有利QTL效应。
Low temperature affects the rice plants at all stages of growth. It can cause severe seedling injury and male sterility resulting in severe yield losses. Using a mini core collection of 174 Chinese rice accessions and 273 SSR markers we investigated cold tolerance at the germination and booting stages, as well as the underlying genetic bases, by association mapping. Two distinct populations, corresponding to subspecies indica and japonica showed evident differences in cold tolerance and its genetic basis. Both subspecies were sensitive to cold stress at both growth stages. However, japonica was more tolerant than indica at all stages as measured by seedling survival and seed setting. There was a low correlation in cold tolerance between the germination and booting stages. Fifty one quantitative trait loci (QTLs) for cold tolerance were dispersed across all 12 chromosomes; 22 detected at the germination stage and 33 at the booting stage. Eight QTLs were identified by at least two of four measures. About 46% of the QTLs represented new loci. The only QTL shared between indica and japonica for the same measure was qLTSSvR6-2 for SSvR. This implied a complicated mechanism of old tolerance between the two subspecies. According to the relative genotypic effect (RGE) of each genotype for each QTL, we detected 18 positive genotypes and 21 negative genotypes in indica, and 19 positive genotypes and 24 negative genotypes in japonica. In general, the negative effects were much stronger than the positive effects in both subspecies. Markers for QTL with positive effects in one subspecies were shown to be effective for selection of cold tolerance in that subspecies, but not in the other subspecies. QTL with strong negative effects on cold tolerance should be avoided during MAS breeding so as to not cancel the effect of favorable QTL at other loci.
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DOI：
10.1073/pnas.0805303105
发表时间：
2008-08-26
影响因子：
11.1
作者：
Fujino, Kenji;Sekiguchi, Hiroshi;Yano, Masahiro
通讯作者：
Yano, Masahiro
影响因子：
2.4
作者：
Dai, LY;Lin, XH;Zhang, DP
通讯作者：
Zhang, DP
影响因子：
7.2
作者：
Flint-Garcia, SA;Thuillet, AC;Buckler, ES
通讯作者：
Buckler, ES
影响因子：
5.4
作者：
Fujino, K;Sekiguchi, H;Yano, M
通讯作者：
Yano, M
影响因子：
1.9
作者：
Cui, Di;Xu, Chang-ying;Han, Long-zhi
通讯作者：
Han, Long-zhi