Biological responses to spider silk-antibiotic fusion protein.
Biological responses to spider silk-antibiotic fusion protein.
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DOI：
10.1002/term.437
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发表时间：
2012-05
影响因子：
3.3
通讯作者：
Kaplan, David L.
中科院分区：
文献类型：
--
作者：
Gomes, Silvia;Gallego-Llamas, Jabier;Leonor, Isabel B.;Mano, Joao F.;Reis, Rui L.;Kaplan, David L.
关键词：
The development of a new generation of multifunctional biomaterials is a continual goal for the field of materials science. The in vivo functional behaviour of a new fusion protein that combines the mechanical properties of spider silk with the antimicrobial properties of hepcidin was addressed in this study. This new chimeric protein, termed 6mer+hepcidin, fuses spider dragline consensus sequences (6mer) and the antimicrobial peptide hepcidin as we have recently described, with retention of bactericidal activity and low cytotoxicity. In the present study mice subcutaneous implants were studied to access the in vivo biological response to the 6mer+hepcidin, which were compared with controls of silk alone (6mer), poly-lactic-glycolic-acid (PLGA) films and empty defects. Along with visual observations, flow cytometry and histology analyses were used to determine the number and type of inflammatory cells at the implantation site. The results show a mild to low inflammatory reaction to the implanted materials and no apparent differences between the 6mer+hepcidin films and the other experimental controls, demonstrating that the new fusion protein has good in vivo biocompatibility, while maintaining antibiotic function.
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影响因子：
6.2
作者：
Bini, Elisabetta;Foo, Cheryl Wong Po;Kaplan, David L.
通讯作者：
Kaplan, David L.
DOI：
10.1111/j.1582-4934.2006.tb00436.x
发表时间：
2006-07
影响因子：
5.3
作者：
Allmeling C;Jokuszies A;Reimers K;Kall S;Vogt PM
通讯作者：
Vogt PM
影响因子：
4
作者：
Chao, KH;Wu, MY;Ho, HN
通讯作者：
Ho, HN
影响因子：
3.9
作者：
Hiemstra, PS
通讯作者：
Hiemstra, PS
影响因子：
4.4
作者：
Grigat, Jasmin;Soruri, Afsaneh;Zwirner, Joerg
通讯作者：
Zwirner, Joerg