CD34+ hematopoietic progenitors from human cord blood differentiate along two independent dendritic cell pathways in response to GM-CSF+TNF alpha.
CD34+ hematopoietic progenitors from human cord blood differentiate along two independent dendritic cell pathways in response to GM-CSF+TNF alpha.
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DOI：
10.1084/jem.184.2.695
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发表时间：
1996-08-01
影响因子：
15.3
通讯作者：
Banchereau, J
中科院分区：
文献类型：
--
作者：
Caux, C;Vanbervliet, B;Massacrier, C;DezutterDambuyant, C;deSaintVis, B;Jacquet, C;Yoneda, K;Imamura, S;Schmitt, D;Banchereau, J
在GM-CSF +TNF α存在下培养CD 34+造血祖细胞12天，可以在体外大量产生人树突状细胞（DC）.目前的研究表明，脐带血CD 34 + HPC确实分化沿着两个独立的DC途径。在培养过程的早期时间点（第5-7天），通过CD 1a和CD 14的排他性表达鉴定的DC前体的两个子集独立地出现。两种前体亚群在第12-14天成熟为具有典型形态和表型（CD 80、CD 83、CD 86、CD 58、高HLA II类）的DC。CD 1a+前体产生以Birbeck颗粒、Lag抗原和E-钙粘蛋白表达为特征的细胞，这三种标记物特异性表达于表皮中的朗格汉斯细胞上。相反，CD 14+祖细胞成熟为CD 1a + DC，缺乏Birbeck颗粒、E-cadherin和Lag抗原，但表达真皮树突状细胞中描述的CD 2、CD 9、CD 68和凝血因子XIIIa。这两种成熟DC在刺激同种异体CD 45 RA+幼稚T细胞方面同样有效。有趣的是，CD 14+前体细胞，而不是CD 1a+前体细胞，代表可以被诱导分化的双能细胞，响应于M-CSF，成巨噬细胞样细胞，缺乏辅助功能的T细胞。总之，这些结果表明存在不同的DC发育途径：朗格汉斯细胞和CD 14（+）-衍生的DC与真皮DC或循环血液DC相关。这两种途径的DC发展的生理相关性进行了讨论，就其潜在的体内同行。
Human dendritic cells (DC) can now be generated in vitro in large numbers by culturing CD34+ hematopoietic progenitors in presence of GM- CSF+TNF alpha for 12 d. The present study demonstrates that cord blood CD34+ HPC indeed differentiate along two independent DC pathways. At early time points (day 5-7) during the culture, two subsets of DC precursors identified by the exclusive expression of CD1a and CD14 emerge independently. Both precursor subsets mature at day 12-14 into DC with typical morphology and phenotype (CD80, CD83, CD86, CD58, high HLA class II). CD1a+ precursors give rise to cells characterized by the expression of Birbeck granules, the Lag antigen and E-cadherin, three markers specifically expressed on Langerhans cells in the epidermis. In contrast, the CD14+ progenitors mature into CD1a+ DC lacking Birbeck granules, E-cadherin, and Lag antigen but expressing CD2, CD9, CD68, and the coagulation factor XIIIa described in dermal dendritic cells. The two mature DC were equally potent in stimulating allogeneic CD45RA+ naive T cells. Interestingly, the CD14+ precursors, but not the CD1a+ precursors, represent bipotent cells that can be induced to differentiate, in response to M-CSF, into macrophage-like cells, lacking accessory function for T cells. Altogether, these results demonstrate that different pathways of DC development exist: the Langerhans cells and the CD14(+)-derived DC related to dermal DC or circulating blood DC. The physiological relevance of these two pathways of DC development is discussed with regard to their potential in vivo counterparts.
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影响因子：
4.4
作者：
Sallusto, Federica;Lanzavecchia, Antonio
通讯作者：
Lanzavecchia, Antonio
DOI：
10.1073/pnas.90.7.3038
发表时间：
1993-04-01
影响因子：
11.1
作者：
INABA, K;INABA, M;STEINMAN, RM
通讯作者：
STEINMAN, RM
影响因子：
64.8
作者：
ARDAVIN, C;WU, L;SHORTMAN, K
通讯作者：
SHORTMAN, K
影响因子：
15.3
作者：
CAUX, C;DURAND, I;BANCHEREAU, J
通讯作者：
BANCHEREAU, J
影响因子：
6.5
作者：
BLAUVELT, A;KATZ, SI;UDEY, MC
通讯作者：
UDEY, MC