Transforming growth factor-beta1 modulates insulin-like growth factor binding protein-4 expression and proteolysis in cultured periosteal explants.
Transforming growth factor-beta1 modulates insulin-like growth factor binding protein-4 expression and proteolysis in cultured periosteal explants.
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DOI：
10.1016/j.ghir.2009.06.002
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发表时间：
2010-04
影响因子：
1.4
通讯作者：
Reinholz, Gregory G.
中科院分区：
文献类型：
--
作者：
Gonzalez, Carlos;Yang, Kiem G. Auw;Schwab, Joseph H.;Fitzsimmons, James S.;Reinholz, Monica M.;Resch, Zachary T.;Bale, Laurie K.;Clemens, Victoria R.;Conover, Cheryl A.;O'Driscoll, Shawn W.;Reinholz, Gregory G.
关键词：
Periosteum is involved in bone growth and fracture healing and has been used as a cell source and tissue graft for tissue engineering and orthopedic reconstruction including joint resurfacing. Periosteum can be induced by transforming growth factor beta (TGF-β) or insulin-like growth factor-I (IGF-I) alone or in combination to form cartilage. However, little is known about the interaction between IGF and TGF-β signaling during periosteal chondrogenesis. The purpose of this study was to determine the effect of TGF-β1 on IGF binding protein-4 (IGFBP-4) and the IGFBP-4 protease pregnancy-associated plasma protein-A (PAPP-A) expression in cultured periosteal explants. Periosteal explants from rabbits were cultured with or without TGF-β1. IGFBP-4 and PAPP-A mRNA levels were determined by real-time quantitative PCR. Conditioned medium was analyzed for IGFBP-4 and PAPP-A protein levels and IGFBP-4 protease activity. TGF-β1-treated explants contained lower IGFBP-4 mRNA levels throughout the culture period with a maximum reduction of 70 % on day 5 of culture. Lower levels of IGFBP-4 protein were also detected in the conditioned medium from TGF-β1-treated explants. PAPP-A mRNA levels were increased 1.6 fold, PAPP-A protein levels were increased 3 fold, and IGFBP-4 protease activity was increased 8.5 fold between 7 and 10 days of culture (the onset of cartilage formation in this model) in conditioned medium from TGF-β1-treated explants. This study demonstrates that TGF-β1 modulates the expression of IGFBP-4 and PAPP-A in cultured periosteal explants.
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影响因子：
2.9
作者：
HOSSENLOPP, P;SEURIN, D;BINOUX, M
通讯作者：
BINOUX, M
影响因子：
--
作者：
Fernihough, JK;Billingham, MEJ;Holly, JMP
通讯作者：
Holly, JMP
影响因子：
7
作者：
Iwanaga, H;Matsumoto, T;Koji, T
通讯作者：
Koji, T
影响因子：
--
作者：
Mardones, RM;Reinholz, GG;O'Driscoll, SW
通讯作者：
O'Driscoll, SW
影响因子：
4.1
作者：
NAKAHARA, H;BRUDER, SP;CAPLAN, AI
通讯作者：
CAPLAN, AI