Histamine activates chloride conductance in motor neurons of the lobster cardiac ganglion.
Histamine activates chloride conductance in motor neurons of the lobster cardiac ganglion.
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组胺激活龙虾心脏神经节运动神经元的氯电导。
DOI：
10.1152/jn.1992.68.1.9
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发表时间：
1992
影响因子：
2.5
通讯作者：
Freschi,JE
中科院分区：
文献类型：
--
作者：
Hashemzadeh-Gargari,H;Freschi,JE
1.用两个微电极对龙虾心神经节的单个运动神经元进行电压钳位。组胺灌流诱发浓度依赖性的膜电流。浓度-效应关系的平均有效浓度（EC 50）为28 μ M。2.组胺激活电流的幅度和极性依赖于细胞内和细胞外Cl-浓度。电流极性反转时的膜电位是Cl-平衡电位的函数。3.组胺激活的Cl-电导是电压依赖性的，随着去极化而增加。因此，组胺诱发的电流表现出外向整流。4.我们的结论是，组胺激活的Cl-电导与生物物理特性类似的甲壳类动物的Cl-电导激活的γ-氨基丁酸（GABA）和组胺的反应中描述的龙虾嗅觉和口胃神经元。5.对组胺的反应被西咪替丁（哺乳动物中的H2亚型抑制剂）竞争性抑制（IC 50 = 7 μ M）。雷尼替丁、吡拉明、扑尔敏、苯海拉明和赛庚啶的效力比西咪替丁低50-100倍。筒箭毒碱是一种Cl-通道阻断剂，其阻断IC 50为20 μ M，但印防己毒素直到浓度超过0.1 mM才开始抑制组胺反应。这些结果表明，不能很容易地使用在哺乳动物中开发的药理学类别进行分类的反应。与许多无脊椎动物对各种神经递质的Cl（-）依赖性反应一样，龙虾心神经节中的组胺反应被筒箭毒碱抑制。7. GABA和组胺对运动神经元的作用相似，但只有GABA抑制起搏脉冲。在这方面，GABA更类似于内源性抑制性突触后电位。（250字处删节）
1. Individual motor neurons of the lobster cardiac ganglion were voltage clamped with two microelectrodes. Superfusion of histamine evoked a concentration-dependent membrane current. The mean effective concentration (EC50) for the concentration-effect relationship was 28 microM. 2. The amplitude and polarity of the histamine-activated current depended on intracellular and extracellular Cl- concentration. The membrane potential at which the current polarity reversed was a function of the Cl- equilibrium potential. 3. The histamine-activated Cl- conductance was voltage dependent, increasing with depolarization. As a consequence, the histamine-evoked current showed outward rectification. 4. We conclude that histamine activates a Cl- conductance with biophysical properties similar to the crustacean Cl- conductance activated by gamma-aminobutyric acid (GABA) and to the histamine responses described in lobster olfactory and stomatogastric neurons. 5. The response to histamine was competitively inhibited (IC50 = 7 microM) by cimetidine, an H2 subtype inhibitor in mammals. Ranitidine, pyrilamine, chlorpheniramine, diphenhydramine, and cyproheptadine were 50-100 times less potent than cimetidine. Tubocurarine, a Cl- channel blocker, blocked with an IC50 of 20 microM, but picrotoxin did not begin to inhibit the histamine response until concentrations exceeded 0.1 mM. 6. These results suggest that the response cannot easily be classified with the use of the pharmacological categories developed in mammals. Like the Cl(-)-dependent responses to various neurotransmitters in a number of invertebrates, the histamine response in the lobster cardiac ganglion was inhibited by tubocurarine. 7. Both GABA and histamine had similar effects on the motor neurons, but only GABA inhibited pacemaker bursts. In this respect, GABA more resembles the endogenous inhibitory postsynaptic potential.(ABSTRACT TRUNCATED AT 250 WORDS)
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