The R2R3-MYB transcription factor gene family in maize.
The R2R3-MYB transcription factor gene family in maize.
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DOI：
10.1371/journal.pone.0037463
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Tang YX
中科院分区：
文献类型：
--
作者：
Du H;Feng BR;Yang SS;Huang YB;Tang YX
MYB蛋白是一个植物转录因子大家族，其成员在植物生物学过程中发挥着多种功能。到目前为止，还没有在玉米(Zea Mays)中对该基因家族进行全基因组特征描述。在本研究中，我们进行了全面的计算分析，以获得玉米R2R3-MYB基因家族的完整概述，包括系统发育、表达模式以及其结构和功能特征。玉米和拟南芥的MYB基因结构高度保守，表明它们原本大小紧凑。MYB结构域以外的亚群特异保守基序可能反映了功能保守。基因组分布有力地支持了分段和串联复制有助于玉米MYB基因扩增的假说。我们还对玉米和其他植物中的R2R3-MYB基因家族进行了更新和全面的分类。结果表明，玉米MYB基因与它们的同源基因之间的功能是保守的，说明了植物MYB基因的起源和进化多样性。物种特有的类群/亚类可能在进化过程中进化或消失，从而导致功能分化。表达谱研究表明，玉米R2R3-MYB基因表现出不同的表达模式，暗示着不同的功能。此外，玉米microRNAs(MiRNAs)的计算预测潜在靶点显示，miR159、miR319和miR160可能参与调控玉米R2R3-myb基因，表明这些miRNAs在转录后调控和转录网络中发挥作用。我们对玉米中R2R3-MYB基因的比较分析证实并扩展了该基因家族的序列和功能特征，为今后玉米MYB基因家族的功能分析奠定了基础。
MYB proteins comprise a large family of plant transcription factors, members of which perform a variety of functions in plant biological processes. To date, no genome-wide characterization of this gene family has been conducted in maize (Zea mays). In the present study, we performed a comprehensive computational analysis, to yield a complete overview of the R2R3-MYB gene family in maize, including the phylogeny, expression patterns, and also its structural and functional characteristics. The MYB gene structure in maize and Arabidopsis were highly conserved, indicating that they were originally compact in size. Subgroup-specific conserved motifs outside the MYB domain may reflect functional conservation. The genome distribution strongly supports the hypothesis that segmental and tandem duplication contribute to the expansion of maize MYB genes. We also performed an updated and comprehensive classification of the R2R3-MYB gene families in maize and other plant species. The result revealed that the functions were conserved between maize MYB genes and their putative orthologs, demonstrating the origin and evolutionary diversification of plant MYB genes. Species-specific groups/subgroups may evolve or be lost during evolution, resulting in functional divergence. Expression profile study indicated that maize R2R3-MYB genes exhibit a variety of expression patterns, suggesting diverse functions. Furthermore, computational prediction potential targets of maize microRNAs (miRNAs) revealed that miR159, miR319, and miR160 may be implicated in regulating maize R2R3-MYB genes, suggesting roles of these miRNAs in post-transcriptional regulation and transcription networks. Our comparative analysis of R2R3-MYB genes in maize confirm and extend the sequence and functional characteristics of this gene family, and will facilitate future functional analysis of the MYB gene family in maize.
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影响因子：
6.9
作者：
Colquhoun TA;Kim JY;Wedde AE;Levin LA;Schmitt KC;Schuurink RC;Clark DG
通讯作者：
Clark DG
影响因子：
7.2
作者：
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9.4
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通讯作者：
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7.4
作者：
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通讯作者：
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影响因子：
9.2
作者：
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通讯作者：
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