Transcriptomics and methylomics in chronic periodontitis with tobacco use: a pilot study.
Transcriptomics and methylomics in chronic periodontitis with tobacco use: a pilot study.
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烟草用途：初步研究慢性牙周炎的转录组学和甲基化组学。
DOI：
10.1186/s13148-017-0381-z
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发表时间：
2017
影响因子：
5.7
通讯作者：
Ryoo HM
中科院分区：
文献类型：
--
作者：
Cho YD;Kim PJ;Kim HG;Seol YJ;Lee YM;Ku Y;Rhyu IC;Ryoo HM
越来越多的证据表明，吸烟影响慢性牙周炎的易感性和严重性。表观遗传学可以解释吸烟在牙周病发生和发展中的作用。在这项研究中，我们进行了转录组和甲基化分析的非牙周炎和牙周炎影响牙龈组织根据吸烟状况。从20名患者中获得人牙龈组织，包括患有和不患有牙周炎的非吸烟者（每组n = 5）和患有和不患有牙周炎的吸烟者（每组n = 5）。分离总RNA和基因组DNA，并根据严格的标准验证其质量。使用Illumina NextSeq 500测序系统生成转录组和甲基化组数据集。综合组间相关性、差异基因表达、DNA甲基化、基因组富集、蛋白质-蛋白质相互作用等分析结果表明，吸烟可能改变了细胞外基质（ECM）组织相关基因的转录和甲基化状态，从而加重了牙周病的病情。我们的研究结果表明，吸烟相关的DNA甲基化模式的变化和随后的ECM组分编码基因表达的改变可能与吸烟者牙周炎易感性增加有因果关系，因为它们可能影响ECM组织，这反过来可能对疾病特征产生影响。本文的在线版本（doi：10.1186/s13148-017-0381-z）包含补充材料，可供授权用户使用。
Accumulating evidence suggests that tobacco smoking affects the susceptibility to and severity of chronic periodontitis. Epigenetics may explain the role of smoking in the development and progress of periodontal disease. In this study, we performed transcriptomic and methylomic analyses of non-periodontitis and periodontitis-affected gingival tissues according to smoking status. Human gingival tissues were obtained from 20 patients, including non-smokers with and without periodontitis (n = 5 per group) and smokers with and without periodontitis (n = 5 per group). Total RNA and genomic DNA were isolated, and their quality was validated according to strict standards. The Illumina NextSeq500 sequencing system was used to generate transcriptome and methylome datasets. Comprehensive analysis, including between-group correlation, differential gene expression, DNA methylation, gene set enrichment, and protein-protein interaction, indicated that smoking may change the transcription and methylation states of extracellular matrix (ECM) organization-related genes, which exacerbated the periodontal condition. Our results suggest that smoking-related changes in DNA methylation patterns and subsequent alterations in the expression of genes coding for ECM components may be causally related to the increased susceptibility to periodontitis in smokers as they could influence ECM organization, which in turn may have an effect on disease characteristics. The online version of this article (doi:10.1186/s13148-017-0381-z) contains supplementary material, which is available to authorized users.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
2.9
作者：
Kubota M;Tanno-Nakanishi M;Yamada S;Okuda K;Ishihara K
通讯作者：
Ishihara K
影响因子：
6.7
作者：
Oliveira, Naila F. P.;Damm, Gilcy R.;de Souza, Ana Paula
通讯作者：
de Souza, Ana Paula
影响因子：
3.5
作者：
Besingi, Welisane;Johansson, Asa
通讯作者：
Johansson, Asa