Water deficit increases stilbene metabolism in Cabernet Sauvignon berries.
Water deficit increases stilbene metabolism in Cabernet Sauvignon berries.
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DOI：
10.1021/jf1024888
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发表时间：
2011-01-12
影响因子：
6.1
通讯作者：
Cramer GR
中科院分区：
文献类型：
--
作者：
Deluc LG;Decendit A;Papastamoulis Y;Mérillon JM;Cushman JC;Cramer GR
研究了水分亏缺对酿酒葡萄(Vitis Vinifera)果实二苯乙烯生物合成的影响。水分亏缺使赤霞珠浆果中反式白藜芦醇(白藜芦醇的糖基化形式)的积累增加了5倍，但在霞多丽中没有增加。同样，水分亏缺显著增加了赤霞珠二苯乙烯前体生物合成相关基因的转录丰度。增加二苯乙烯合成酶的表达，但不增加白藜芦醇-O-糖基转移酶的表达，导致反式-白藜芦醇浓度增加。相比之下，霞多丽中相同基因的转录丰度因水分不足而下降。在赤霞珠、霞多丽和黑比诺的二苯乙烯合成酶基因启动子中发现了12个单核苷酸多态(SNPs)。这些多态导致了赤霞珠和霞多丽中预测的顺式调控元件的八个变化。这些结果表明，葡萄中白藜芦醇的生物合成可能存在品种特异性的分子机制。
The impact of water deficit on stilbene biosynthesis in wine grape (Vitis vinifera) berries was investigated. Water deficit increased the accumulation of trans-piceid (the glycosylated form of resveratrol) by 5-fold in Cabernet Sauvignon berries but not in Chardonnay. Similarly, water deficit significantly increased the transcript abundance of genes involved in the biosynthesis of stilbene precursors in Cabernet Sauvignon. Increased expression of stilbene synthase, but not that of resveratrol-O-glycosyltransferase, resulted in increased trans-piceid concentrations. In contrast, the transcript abundance of the same genes declined in Chardonnay in response to water deficit. Twelve single nucleotide polymorphisms (SNPs) were identified in the promoters of stilbene synthase genes of Cabernet Sauvignon, Chardonnay, and Pinot Noir. These polymorphisms resulted in eight changes within the predicted cis regulatory elements in Cabernet Sauvignon and Chardonnay. These results suggest that cultivar-specific molecular mechanisms might exist that control resveratrol biosynthesis in grapes.
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影响因子：
4.4
作者：
Deluc LG;Grimplet J;Wheatley MD;Tillett RL;Quilici DR;Osborne C;Schooley DA;Schlauch KA;Cushman JC;Cramer GR
通讯作者：
Cramer GR
影响因子：
7
作者：
Howe, KL;Chothia, T;Durbin, R
通讯作者：
Durbin, R
影响因子：
7.2
作者：
Hall, Dawn;De Luca, Vincenzo
通讯作者：
De Luca, Vincenzo
影响因子：
64.8
作者：
Jaillon, Olivier;Aury, Jean-Marc;Wincker, Patrick
通讯作者：
Wincker, Patrick
影响因子：
6.1
作者：
Gatto, Pamela;Vrhovsek, Urska;Velasco, Riccardo
通讯作者：
Velasco, Riccardo