A Primer for Single-Cell Sequencing in Non-Model Organisms.

A Primer for Single-Cell Sequencing in Non-Model Organisms.
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DOI:
10.3390/genes13020380
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发表时间:
2022-02-19
期刊:
影响因子:
3.5
通讯作者:
Athrey GN
Athrey GN
中科院分区:
生物学3区
文献类型:
--
作者:
Alfieri JM;Wang G;Jonika MM;Gill CA;Blackmon H;Athrey GN

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单细胞测序技术导致了我们对细胞类型多样性、生物组织水平之间的联系以及基因型和表型之间关系的认识的革命。这些进步主要来自模式生物的使用;然而,在非模式生物中使用单细胞测序可以使研究典型模式生物无法解决的问题成为可能。本引物描述了单细胞测序研究的一般工作流程和使用非模式生物(仅限于多细胞动物)的考虑。重要的是,单细胞测序,当进一步应用于非模式生物时,将允许更深入地了解基因型和表型之间的机制以及生物变异的基础。
Single-cell sequencing technologies have led to a revolution in our knowledge of the diversity of cell types, connections between biological levels of organization, and relationships between genotype and phenotype. These advances have mainly come from using model organisms; however, using single-cell sequencing in non-model organisms could enable investigations of questions inaccessible with typical model organisms. This primer describes a general workflow for single-cell sequencing studies and considerations for using non-model organisms (limited to multicellular animals). Importantly, single-cell sequencing, when further applied in non-model organisms, will allow for a deeper understanding of the mechanisms between genotype and phenotype and the basis for biological variation.
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