A 40-bp A/T-rich repressor element involved in organ-dependent transcriptional regulation of ZmGLU1
A 40-bp A/T-rich repressor element involved in organ-dependent transcriptional regulation of ZmGLU1
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一种富含 A/T 的 40 bp 阻遏元件，参与 ZmGLU1 的器官依赖性转录调节
DOI：
10.1007/s11240-010-9867-5
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发表时间：
2011-06
影响因子：
3
通讯作者：
Zhao,L.
中科院分区：
文献类型：
--
作者：
Gu,R.;Yuan,L.;Zhao,L.
玉米β-葡萄糖苷酶（ZmGLU1）可水解细胞分裂素偶联物释放游离细胞分裂素，调控其表达对植物生长发育过程中细胞分裂素稳态的控制至关重要。虽然ZmGLU1基因的启动子被发现赋予其器官依赖性表达，但其潜在的机制仍未被揭示。在这项研究中，通过启动子缺失实验，在ZmGLU1启动子区域发现了一个40-bp的a / t丰富序列，范围在−798到−758 （T40）之间，介导了种子和地上部启动子活性的抑制，但在根中没有。在花椰菜花叶病毒35S启动子（35S）上游添加T40，抑制了35S驱动基因在茎部和种子中的表达。电泳迁移位移分析进一步揭示了T40与一种未知结合蛋白在种子和茎中存在特异性相互作用，而在根中不存在。ZmGLU1基因表达/启动子活性与T40蛋白相互作用强度呈负相关，提示T40作为抑制因子顺式元件参与ZmGLU1器官依赖性表达。此外，该结果还提供了一个在高等植物中具有转录抑制作用的富含a / t的新序列。
Maize β-glucosidase (ZmGLU1) hydrolyzes cytokinin conjugates to release free cytokinins, and regulation of its expression is essential to control cytokinin homeostasis during plant growth and development. Although the promoter of ZmGLU1 gene was found to confer its organ-dependent expression, the underlying mechanism remained to be uncovered. In this study, by promoter deletion assay, a 40-bp A/T-rich sequence in ZmGLU1 promoter region ranging from −798 to −758 (T40) was identified as mediating the repression of promoter activity in seeds and shoot but not in root. Additional fusion of T40 upstream of the constitutive cauliflower mosaic virus 35S promoter (35S) suppressed 35S-driven gene expression in shoot and seeds. Electrophoretic mobility shift assay further revealed a specific interaction between T40 and an unknown binding protein in seeds and shoot but not in root. The negative correlation between ZmGLU1 gene expression/promoter activity and the intensity of T40–protein interaction suggests that T40 acts as a repressor cis-element involved in ZmGLU1 organ-dependent expression. Furthermore, this result provides a new A/T-rich sequence exhibiting transcriptional repression in higher plant.
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影响因子：
56.9
作者：
BRZOBOHATY, B;MOORE, I;PALME, K
通讯作者：
PALME, K
影响因子：
5.4
作者：
Angulo, A;Kerry, D;Ghazal, P
通讯作者：
Ghazal, P
影响因子：
7.2
作者：
Pyo, Hyunjin;Demura, Taku;Fukuda, Hiroo
通讯作者：
Fukuda, Hiroo
影响因子：
4.8
作者：
Berry, FB;Miura, Y;Tamaoki, T
通讯作者：
Tamaoki, T
影响因子：
12
作者：
S. Davis
通讯作者：
S. Davis