Dietary Alaska Pollack Protein Induces Acute and Sustainable Skeletal Muscle Hypertrophy in Rats.
Dietary Alaska Pollack Protein Induces Acute and Sustainable Skeletal Muscle Hypertrophy in Rats.
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DOI：
10.3390/nu14030547
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发表时间：
2022-01-27
期刊：
影响因子：
5.9
通讯作者：
Kishida T
中科院分区：
文献类型：
--
作者：
Uchida K;Fujitani M;Mizushige T;Kawabata F;Hayamizu K;Uozumi K;Hara Y;Sawai M;Uehigashi R;Okada S;Goto-Inoue N;Morisasa M;Kishida T
我们之前的研究表明，摄入阿拉斯加鳕鱼蛋白(APP)会增加骨骼肌质量，并可能导致喂食高脂饮食56天的大鼠肌肉纤维类型从慢到快的转变。在这项研究中，我们探索了饮食APP是否会诱导喂养正常脂肪饮食的大鼠急性和可持续的骨骼肌肥大。将5周龄雄性SD大鼠分为4组，分别饲喂含等量粗蛋白的高脂饲料或添加酪蛋白或APP的高脂饲料。饲喂APP后2、7天，无论饲料脂肪含量如何，腓肠肌质量均显著增加(105~110%)。将大鼠分成两组，分别饲喂含有酪蛋白或APP的普通脂肪饲料。饲粮APP显著增加了56d仔猪的腓肠肌质量(110%)。饲喂APP 7天后，大鼠腓肠肌和富胶原结缔组织的横截面积显著增加。7d后可降低MSTN/Myostatin、Trim63/MuRF1、Fbxo32/ATROGIN-1等基因的表达，但不影响血清IGF-1等其他基因的表达。饲喂7天后，肌球蛋白ATPase染色显示，酪蛋白组和APP组之间I型、IIA型和IIB型纤维的百分比没有差异。在相似的实验中，饲喂2天后，饲粮酪蛋白和APP的嘌呤霉素标记的多肽没有差异。这些结果表明，无论饮食中的脂肪含量如何，APP都能诱导大鼠急性和可持续的骨骼肌肥大。膳食APP作为一种日常蛋白质来源，可能是维持或增加肌肉质量的一种方法。
Our previous studies suggested that Alaska pollack protein (APP) intake increases skeletal muscle mass and that it may cause a slow-to-fast shift in muscle fiber type in rats fed a high-fat diet after 56 days of feeding. In this study, we explored whether dietary APP induces acute and sustainable skeletal muscle hypertrophy in rats fed a normal-fat diet. Male 5-week-old Sprague–Dawley rats were divided into four groups and fed a purified ingredient-based high-fat diet or a purified ingredient-based normal-fat diet with casein or APP, containing the same amount of crude protein. Dietary APP significantly increased gastrocnemius muscle mass (105~110%) after 2, 7 days of feeding, regardless of dietary fat content. Rats were separated into two groups and fed a normal-fat diet with casein or APP. Dietary APP significantly increased gastrocnemius muscle mass (110%) after 56 days of feeding. Dietary APP significantly increased the cross-sectional area of the gastrocnemius skeletal muscle and collagen-rich connective tissue after 7 days of feeding. It decreased the gene expression of Mstn /Myostatin, Trim63/MuRF1, and Fbxo32/atrogin-1, but not other gene expression, such as serum IGF-1 after 7 days of feeding. No differences were observed between casein and APP groups with respect to the percentage of Type I, Type IIA, and Type IIX or IIB fibers, as determined by myosin ATPase staining after 7 days of feeding. In the similar experiment, the puromycin-labeled peptides were not different between dietary casein and APP after 2 days of feeding. These results demonstrate that APP induces acute and sustainable skeletal muscle hypertrophy in rats, regardless of dietary fat content. Dietary APP, as a daily protein source, may be an approach for maintaining or increasing muscle mass.
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影响因子：
4.6
作者：
Le NH;Kim CS;Park T;Park JH;Sung MK;Lee DG;Hong SM;Choe SY;Goto T;Kawada T;Yu R
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作者：
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作者：
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通讯作者：
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