Prevaccination Glycan Markers of Response to an Influenza Vaccine Implicate the Complement Pathway.
Prevaccination Glycan Markers of Response to an Influenza Vaccine Implicate the Complement Pathway.
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DOI：
10.1021/acs.jproteome.2c00251
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发表时间：
2022-08-05
影响因子：
4.4
通讯作者：
Mahal, Lara K.
中科院分区：
文献类型：
--
作者：
Qin, Rui;Meng, Guanmin;Pushalkar, Smruti;Carlock, Michael A.;Ross, Ted M.;Vogel, Christine;Mahal, Lara K.
理解疫苗应答随宿主因素变化的机制是改进疫苗设计和疫苗接种策略的关键。糖基化是一种重要的免疫调节剂，在决定疫苗应答方面没有明确的作用。为了深入了解糖基化与疫苗诱导的抗体水平之间的关系，我们使用凝集素微阵列技术分析了2019-2020年流感季节期间接受FLUZONE流感疫苗的160名高加索成年人的疫苗接种前和接种后血清蛋白糖组。我们发现，接种前水平的刘易斯A抗原（莱亚）是显着高于无反应者。糖蛋白质组学分析表明，Lea承载蛋白质富含补体激活途径，表明糖基化在调节补体蛋白活性中的潜在作用，这可能与安装疫苗应答有关。此外，我们观察到免疫后高应答者唾液酸刘易斯X抗原（sLex）增加，高甘露糖聚糖减少，而无应答者则没有观察到。这些数据表明，免疫系统可能主动调节糖基化，作为其建立有效保护接种后的努力的一部分。
A key to improving vaccine design and vaccination strategy is to understand the mechanism behind the variation of vaccine response with host factors. Glycosylation, a critical modulator of immunity, has no clear role in determining vaccine responses. To gain insight into the association between glycosylation and vaccine-induced antibody levels, we profiled the pre- and postvaccination serum protein glycomes of 160 Caucasian adults receiving the FLUZONE influenza vaccine during the 2019–2020 influenza season using lectin microarray technology. We found that prevaccination levels of Lewis A antigen (Lea) are significantly higher in nonresponders than responders. Glycoproteomic analysis showed that Lea-bearing proteins are enriched in complement activation pathways, suggesting a potential role of glycosylation in tuning the activities of complement proteins, which may be implicated in mounting vaccine responses. In addition, we observed a postvaccination increase in sialyl Lewis X antigen (sLex) and a decrease in high mannose glycans among high responders, which were not observed in nonresponders. These data suggest that the immune system may actively modulate glycosylation as part of its effort to establish effective protection postvaccination.
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影响因子：
14.9
作者：
Gene Ontology Consortium
通讯作者：
Gene Ontology Consortium
影响因子：
5
作者：
Giampaoli O;Conta G;Calvani R;Miccheli A
通讯作者：
Miccheli A
影响因子：
4
作者：
Bojar, Daniel;Meche, Lawrence;Meng, Guanmin;Eng, William;Smith, David F.;Cummings, Richard D.;Mahal, Lara K.
通讯作者：
Mahal, Lara K.
影响因子：
4.3
作者：
Martin, Myriam;Gottsater, Anders;Blom, Anna M.
通讯作者：
Blom, Anna M.
刘易斯表型是尼加拉瓜儿童RotateQ和Rotarix疫苗接种的限制因素。
影响因子：
4.6
作者：
Bucardo F;Nordgren J;Reyes Y;Gonzalez F;Sharma S;Svensson L
通讯作者：
Svensson L