Uptake and reversibility of uptake of nickel by human macrophages.
Uptake and reversibility of uptake of nickel by human macrophages.
复制标题
人类巨噬细胞对镍的摄取和摄取的可逆性。
DOI：
10.1046/j.1365-2842.1998.00197.x
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发表时间：
1998
影响因子：
2.9
通讯作者：
C. T. Hanks
中科院分区：
文献类型：
--
作者：
D. L. Edwards;J. Wataha;C. T. Hanks
由于镍的生物责任和镍离子从牙科器械释放到口腔中，含镍合金在牙科中的使用受到了质疑。口腔组织中细胞对镍的摄取是评估镍的生物易感性的一个关键因素。巨噬细胞的镍摄取可能特别重要，因为巨噬细胞在炎症过程中的中心作用，以及已知的巨噬细胞在协调对生物材料的反应中的作用。本研究的目的是评估人类巨噬细胞摄取镍的可逆性，并确定镍到达细胞核的比例随时间的变化。用原子吸收光谱法测定细胞内镍含量。分离细胞后测定细胞核镍含量。结果表明，巨噬细胞能迅速摄取镍，并在8h内在胞核内蓄积，48h后，60%以上的细胞镍进入胞核。一旦被吸收，镍的损失速度就会更低。随着初始暴露于镍的时间的增加，损失率下降。因此，结果表明，如果暴露之间的时间不足以逆转摄取镍的情况，巨噬细胞可能会积累镍。有必要进一步研究镍的滞留与巨噬细胞功能受损之间的关系，并进一步确定在牙科合金中使用镍的生物学风险。
The use of nickel-containing alloys in dentistry has been questioned because of the biological liabilities of nickel and the release of nickel ions from dental appliances into the oral cavity. The uptake of nickel by cells in the oral tissues is a critical factor in assessing the biological liabilities of nickel. Nickel uptake by macrophages may be particularly important because of the central role of macrophages in the inflammatory process and the known role of the macrophage in orchestrating the response to biomaterials. The aims of the current study were to assess the reversibility of the uptake of nickel from human macrophages and determine the portion of the nickel which reaches the nuclei as a function of time. Cellular nickel content was measured by means of atomic absorption spectrometry. Nuclear nickel content was assessed after fractionating cells. The results showed that nickel was rapidly taken up by macrophages and that the nickel accumulated in the nucleus in as little as 8 h. After 48 h, over 60% of the cellular nickel was in the nucleus. Once taken up, the nickel was lost at a lower rate. The rate of loss decreased as the initial exposure time to the nickel increased. Thus, the results indicated that macrophages may accumulate nickel if the time between exposures is insufficient to reverse the uptake. Further studies are necessary to correlate the retention of nickel with impaired function of macrophages and to further define the biological risks of using nickel in dental alloys.
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从体外致癌模型中获得对镍致癌机制的看法。
影响因子：
10.4
作者：
Costa,M
通讯作者：
Costa,M
体外成纤维细胞对金属阳离子的摄取。
DOI：
10.1002/jbm.820270212
发表时间：
1993
期刊：
Journal of biomedical materials research
影响因子：
--
作者：
Wataha,JC;Hanks,CT;Craig,RG
通讯作者：
Craig,RG
影响因子：
3.5
作者：
PAGE, RC
通讯作者：
PAGE, RC
DOI：
10.1002/jbm.820280404
发表时间：
1994-04
期刊：
Journal of biomedical materials research
影响因子：
--
作者：
J. Wataha;C. T. Hanks;R. G. Craig
通讯作者：
J. Wataha;C. T. Hanks;R. G. Craig
影响因子：
14
作者：
ANDERSON, JM;MILLER, KM
通讯作者：
MILLER, KM