Protist-type lysozymes of the nematode Caenorhabditis elegans contribute to resistance against pathogenic Bacillus thuringiensis.
Protist-type lysozymes of the nematode Caenorhabditis elegans contribute to resistance against pathogenic Bacillus thuringiensis.
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DOI：
10.1371/journal.pone.0024619
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Schulenburg H
中科院分区：
文献类型：
--
作者：
Boehnisch C;Wong D;Habig M;Isermann K;Michiels NK;Roeder T;May RC;Schulenburg H
病原体对其他生物体构成普遍威胁。大多数生物体表达抗菌蛋白和肽，例如溶菌酶，作为抵御这些挑战的保护措施。线虫秀丽隐杆线虫拥有 15 个系统发育多样化的溶菌酶基因，属于两种不同的类型：原生生物或内阿米巴型（lys 基因）和无脊椎动物型（ilys 基因）溶菌酶。在本研究中，我们描述了几种原生型溶菌酶基因在防御革兰氏阳性细菌苏云金芽孢杆菌杀线虫菌株中的作用。基于微阵列和随后的 qRT-PCR 基因表达分析，我们将原生生物型溶菌酶基因鉴定为感染后差异转录的基因类别之一。对其中三个基因进行了功能遗传分析，每个基因都属于原生生物型溶菌酶（lys-2、lys-5 和 lys-7）内的不同进化谱系。它们的敲除导致所有三种情况下的病原体抗性下降，而当转基因系中三分之二的测试基因过表达时（lys-5、lys-7，但不是 lys-2），观察到抗性增加。我们得出的结论是，溶菌酶基因 lys-5、lys-7 和可能的 lys-2 有助于抵抗苏云金芽孢杆菌，从而强调了溶菌酶在线虫防御病原体中的特殊作用。
Pathogens represent a universal threat to other living organisms. Most organisms express antimicrobial proteins and peptides, such as lysozymes, as a protection against these challenges. The nematode Caenorhabditis elegans harbours 15 phylogenetically diverse lysozyme genes, belonging to two distinct types, the protist- or Entamoeba-type (lys genes) and the invertebrate-type (ilys genes) lysozymes. In the present study we characterized the role of several protist-type lysozyme genes in defence against a nematocidal strain of the Gram-positive bacterium Bacillus thuringiensis. Based on microarray and subsequent qRT-PCR gene expression analysis, we identified protist-type lysozyme genes as one of the differentially transcribed gene classes after infection. A functional genetic analysis was performed for three of these genes, each belonging to a distinct evolutionary lineage within the protist-type lysozymes (lys-2, lys-5, and lys-7). Their knock-out led to decreased pathogen resistance in all three cases, while an increase in resistance was observed when two out of three tested genes were overexpressed in transgenic lines (lys-5, lys-7, but not lys-2). We conclude that the lysozyme genes lys-5, lys-7, and possibly lys-2 contribute to resistance against B. thuringiensis, thus highlighting the particular role of lysozymes in the nematode's defence against pathogens.
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影响因子：
4.8
作者：
Ideo, Hiroko;Fukushima, Keiko;Yamashita, Katsuko
通讯作者：
Yamashita, Katsuko
影响因子：
4.8
作者：
Griffitts, JS;Huffman, DL;Aroian, RV
通讯作者：
Aroian, RV
影响因子：
10.7
作者：
Haber, M;Schüngel, M;Schulenburg, H
通讯作者：
Schulenburg, H
影响因子：
4.8
作者：
Hasshoff, Martin;Boehnisch, Claudia;Schulenburg, Hinrich
通讯作者：
Schulenburg, Hinrich
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y