A Novel Minidumbbell DNA-Based Sensor for Silver Ion Detection.

A Novel Minidumbbell DNA-Based Sensor for Silver Ion Detection.
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DOI:
10.3390/bios13030358
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发表时间:
2023-03-08
期刊:
Biosensors
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--
通讯作者:
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其他
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银离子(Ag+)是最常见的造成环境污染和影响人类健康的重金属离子之一,因此,其检测在分析化学领域具有重要意义。在这里,我们报告了一种用于 Ag+ 检测的 8 核苷酸 (nt) 迷你哑铃 DNA 传感器 (M-DNA)。小哑铃的小沟内含有一个独特的反向摆动 C·C 错配,作为 Ag+ 的结合位点。 M-DNA传感器的检测限可达2.1 nM,能够高精度地检测真实环境样品中的Ag+。更重要的是,由于使用超短寡核苷酸,M-DNA传感器表现出快速动力学和易于操作的优点。迷你哑铃代表了一种新的、最小的用于 Ag+ 传感的非 B DNA 结构基序,允许进一步开发现场环境 Ag+ 检测设备。
Silver ion (Ag+) is one of the most common heavy metal ions that cause environmental pollution and affect human health, and therefore, its detection is of great importance in the field of analytical chemistry. Here, we report an 8-nucleotide (nt) minidumbbell DNA-based sensor (M-DNA) for Ag+ detection. The minidumbbell contained a unique reverse wobble C·C mispair in the minor groove, which served as the binding site for Ag+. The M-DNA sensor could achieve a detection limit of 2.1 nM and sense Ag+ in real environmental samples with high accuracy. More importantly, the M-DNA sensor exhibited advantages of fast kinetics and easy operation owing to the usage of an ultrashort oligonucleotide. The minidumbbell represents a new and minimal non-B DNA structural motif for Ag+ sensing, allowing for the further development of on-site environmental Ag+ detection devices.
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