Acupuncture activates signal transduction pathways related to brain-tissue restoration after ischemic injury.
Acupuncture activates signal transduction pathways related to brain-tissue restoration after ischemic injury.
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DOI：
10.3969/j.issn.1673-5374.2012.24.004
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发表时间：
2012-08-25
影响因子：
6.1
通讯作者：
Chen C
中科院分区：
文献类型：
--
作者：
Tian H;Zhang H;Zhu J;Zhang J;Cai H;Zhang Y;Chen C
采用改进的线栓塞法建立大鼠大脑中动脉闭塞模型。对大鼠进行针刺大椎（DU14）、任中（DU26）、百会（DU20）或非经络穴位的治疗。蛋白芯片技术检测显示，与未接受针灸的模型组相比，两组蛋白磷酸化水平均根据信号通路上调或下调。对显示磷酸化下调的蛋白质的分析显示，针灸治疗组有 5 条信号通路被激活，而针灸对照组只有 2 条信号通路被激活。相反，对显示磷酸化上调的蛋白质的分析显示，针灸治疗组中仅激活了一种途径，而针灸对照组中则激活了四种途径。此外，针刺治疗组的激活蛋白数量不仅高于针刺对照组，而且与针刺对照组不同的是，大多数激活蛋白是信号通路中的关键蛋白。我们的研究结果表明，针灸特定穴位可以激活多种信号通路，促进缺血性损伤后脑组织的恢复，这是基于多种通路、靶点和手段的综合作用。
A middle cerebral artery occlusion-model was established in rats using the improved thread embolism method. Rats were treated with acupuncture at either Dazhui (DU14), Renzhong (DU26), Baihui (DU20), or a non-meridian point. Detection with protein-chip technology showed that the level of protein phosphorylation in both groups was upregulated or downregulated depending on the signaling pathway compared with the model group that did not receive acupuncture. Analysis of proteins showing downregulated phosphorylation revealed that five signaling pathways were activated in the acupuncture-treatment group, while only two were activated in the acupuncture- control group. In contrast, analysis of proteins showing upregulated phosphorylation revealed only one pathway was activated in the acupuncture-treatment group, whereas four pathways were activated in the acupuncture-control group. Furthermore, the number of activated proteins in the acupuncture-treatment group was not only higher than the acupuncture-control group, but unlike the acupuncture-control group, the majority of activated proteins were key proteins in the signaling pathways. Our findings indicate that acupuncture at specific points can activate multiple signaling pathways to promote the restoration of brain tissue following ischemic injury, and that this is based on a combination of effects resulting from multiple pathways, targets, and means.
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