Computational analyses of mammalian lactate dehydrogenases: human, mouse, opossum and platypus LDHs.
Computational analyses of mammalian lactate dehydrogenases: human, mouse, opossum and platypus LDHs.
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DOI：
10.1016/j.compbiolchem.2009.07.006
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发表时间：
2009-10
影响因子：
3.1
通讯作者：
Goldberg, Erwin
中科院分区：
文献类型：
--
作者：
Holmes, Roger S.;Goldberg, Erwin
利用基因组序列数据库，采用计算方法预测哺乳动物乳酸脱氢酶(LDH)基因和蛋白质的氨基酸序列和基因位置。人LDHA、LDHC和LDH6A基因位于11号染色体上，LDH6B和LDH6C基因分别位于第15和12号染色体上。负鼠LDHC和LDH6B基因与负鼠LDHA基因位于第5染色体上，含有7个(LDHA和LDHC)或8个(LDH6B)外显子。对负鼠LDH6B亚基的氨基酸序列预测给出了一个延长的N-末端序列，与人和小鼠的LDH6B序列相似，这可能支持该酶输出到线粒体。鸭嘴兽基因组至少含有3个LDH基因，编码LDHA、LDHB和LDH6B亚基。系统发育研究和序列分析表明，LDHA、LDHB和LDH6B基因在所有被研究的哺乳动物基因组中都存在，包括单孔动物(鸭嘴兽)，而LDHC基因可能是最近在有袋类哺乳动物中出现的。
Computational methods were used to predict the amino acid sequences and gene locations for mammalian lactate dehydrogenase (LDH) genes and proteins using genome sequence databanks. Human LDHA, LDHC and LDH6A genes were located in tandem on chromosome 11, while LDH6B and LDH6C genes were on chromosomes 15 and 12, respectively. Opossum LDHC and LDH6B genes were located in tandem with the opossum LDHA gene on chromosome 5 and contained 7 (LDHA and LDHC) or 8 (LDH6B) exons. An amino acid sequence prediction for the opossum LDH6B subunit gave an extended N-terminal sequence, similar to the human and mouse LDH6B sequences, which may support the export of this enzyme into mitochondria. The platypus genome contained at least 3 LDH genes encoding LDHA, LDHB and LDH6B subunits. Phylogenetic studies and sequence analyses indicated that LDHA, LDHB and LDH6B genes are present in all mammalian genomes examined, including a monotreme species (platypus), whereas the LDHC gene may have arisen more recently in marsupial mammals.
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影响因子：
3.5
作者：
HOLMES, RS
通讯作者：
HOLMES, RS
影响因子：
--
作者：
GOLDBERG, E;HAWTREY, C
通讯作者：
HAWTREY, C
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.
影响因子：
14.9
作者：
Chenna, R;Sugawara, H;Thompson, JD
通讯作者：
Thompson, JD
DOI：
10.1073/pnas.84.15.5311
发表时间：
1987-08-01
影响因子：
11.1
作者：
MILLAN, JL;DRISCOLL, CE;GOLDBERG, E
通讯作者：
GOLDBERG, E