Lateral transduction is inherent to the life cycle of the archetypical Salmonella phage P22.
Lateral transduction is inherent to the life cycle of the archetypical Salmonella phage P22.
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DOI：
10.1038/s41467-021-26520-4
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发表时间：
2021-11-08
影响因子：
16.6
通讯作者：
Penadés JR
中科院分区：
文献类型：
--
作者：
Fillol-Salom A;Bacigalupe R;Humphrey S;Chiang YN;Chen J;Penadés JR
溶原诱导结束了噬菌体与其宿主之间的稳定结合，并且向裂解周期的过渡开始于早期原噬菌体切除，随后是DNA复制和包装（ERP）。这种时间程序被认为是普遍的P22样温带的，虽然没有直接的证据来支持这些事件的时间和顺序。在这里，我们报告说，长期的ERP程序是一个观察实验青睐沙门氏菌噬菌体P22 tsc229热诱导突变体，和野生型P22实际上遵循复制包装切除（RPE）程序。我们发现P22 tsc229在诱导后早期切除，但P22将切除延迟到对噬菌体产生有害之前。这允许P22参与横向转导。因此，P22以最小的代价调整了切除的时机，以平衡繁殖与横向转导，通过基因转移为其宿主的进化提供动力，以达到自我保护的目的。在从溶原性（噬菌体与其细菌宿主之间的稳定结合）向溶解周期的过渡期间，原噬菌体切除可以在DNA复制和包装之后或之前进行。在这里，作者表明，原噬菌体切除在沙门氏菌噬菌体P22中被延迟，从而允许通过侧向转导包装和转移宿主DNA的大片段。
Lysogenic induction ends the stable association between a bacteriophage and its host, and the transition to the lytic cycle begins with early prophage excision followed by DNA replication and packaging (ERP). This temporal program is considered universal for P22-like temperate phages, though there is no direct evidence to support the timing and sequence of these events. Here we report that the long-standing ERP program is an observation of the experimentally favored Salmonella phage P22 tsc229 heat-inducible mutant, and that wild-type P22 actually follows the replication-packaging-excision (RPE) program. We find that P22 tsc229 excises early after induction, but P22 delays excision to just before it is detrimental to phage production. This allows P22 to engage in lateral transduction. Thus, at minimal expense to itself, P22 has tuned the timing of excision to balance propagation with lateral transduction, powering the evolution of its host through gene transfer in the interest of self-preservation. During the transition from lysogeny (a stable association between a phage and its bacterial host) to the lytic cycle, prophage excision can be followed or preceded by DNA replication and packaging. Here, the authors show that prophage excision is delayed in Salmonella phage P22, thus allowing the packaging and transfer of large fragments of host DNA via lateral transduction.
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DOI：
10.1038/ismej.2016.163
发表时间：
2017-04
期刊：
The ISME journal
影响因子：
--
作者：
Martínez-Rubio R;Quiles-Puchalt N;Martí M;Humphrey S;Ram G;Smyth D;Chen J;Novick RP;Penadés JR
通讯作者：
Penadés JR
DOI：
10.1073/pnas.1309739110
发表时间：
2013-07-16
影响因子：
11.1
作者：
Liu, Xiaoqiu;Jiang, Huifeng;Roberts, Jeffrey W.
通讯作者：
Roberts, Jeffrey W.
影响因子：
56.9
作者：
LEVINE, M;SMITH, HO
通讯作者：
SMITH, HO
影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL