In budding yeast, reactive oxygen species induce both RAS‐dependent and RAS‐independent cell cycle‐specific arrest
In budding yeast, reactive oxygen species induce both RAS‐dependent and RAS‐independent cell cycle‐specific arrest
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在出芽酵母中，活性氧诱导 RAS 依赖性和 RAS 独立性细胞周期特异性停滞
作者：
Valeria Wanke;Katia Accorsi;D. Porro;F. Esposito;T. Russo;M. Vanoni
马来酸二乙酯 (DEM) 诱导的谷胱甘肽消耗引起的轻度氧化应激在酵母细胞周期进展中的作用已被研究。我们发现不同的野生型菌株对相似 DEM 剂量诱导的氧化应激敏感：YPD 平板上 ≈ 1 mM，摇瓶中 5–10 mM。在较低剂量下，DEM 导致生长速率短暂下降，这主要是因为 G1 到 S 的转变减少。使用较高剂量的 DEM 处理会导致生长完全停滞，大多数细胞处于细胞周期未出芽的 G1 期。 DEM 治疗导致应激反应元件 (STRE) 控制基因的转录诱导，并通过抗氧化剂 N-乙酰半胱氨酸治疗缓解。 cdc25和cdc28突变体的相互转换实验表明，主要的细胞周期停滞点位于起始区域，在CDC25介导的步骤处或附近，在CDC28细胞周期蛋白依赖性激酶介导的步骤之前。 DEM 诱导的 G1 停滞需要适当调节的 RAS 通路，并且可以通过过度表达 G1 特异性细胞周期蛋白 CLN2 来绕过。然而，RAS 途径失调或 CLN2 过度表达的细胞无法生长并被抑制为出芽细胞，这表明存在第二个 DEM 敏感细胞周期步骤。
The role of mild oxidative stresses elicited by diethylmaleate (DEM)‐induced glutathione depletion in the progression of the yeast cell cycle has been investigated. We found that different wild‐type strains are sensitive to oxidative stresses induced by similar DEM doses: ≈ 1 mM on YPD plates, 5–10 mM in shaken flasks. At lower doses, DEM caused a transient decrease in growth rate, largely because of a decreased G1‐to‐S transition. Treatment with higher DEM doses leads to complete growth arrest, with most cells found in the unbudded G1 phase of the cell cycle. DEM treatment resulted in transcriptional induction of stress‐responsive element (STRE)‐controlled genes and was relieved by treatment with the antioxidant N‐acetyl cysteine. Reciprocal shift experiments with cdc25 and cdc28 mutants showed that the major cell cycle arrest point was located in the Start area, at or near the CDC25‐mediated step, before the step mediated by the CDC28 cyclin‐dependent kinase. The DEM‐induced G1 arrest requires a properly regulated RAS pathway and can be bypassed by overexpressing the G1‐specific cyclin CLN2. However, cells with either a deregulated RAS pathway or overexpressing CLN2 failed to grow and arrested as budded cells, indicating that a second DEM‐sensitive cell cycle step exists.
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影响因子：
37.8
作者：
Badimon,JJ;Fuster,V;Chesebro,JH;Badimon,L
通讯作者：
Badimon,L
影响因子：
3.3
作者：
E. Muller
通讯作者：
E. Muller
选择细胞分裂起始事件有缺陷的酿酒酵母突变体。
影响因子：
3.3
作者：
Reed,SI
通讯作者：
Reed,SI
将其插入：信号电路和酵母细胞周期。
影响因子：
7.5
作者：
Wittenberg,C;Reed,SI
通讯作者：
Reed,SI