Photoperiodic regulation of the orexigenic effects of ghrelin in Siberian hamsters.
Photoperiodic regulation of the orexigenic effects of ghrelin in Siberian hamsters.
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DOI：
10.1016/j.yhbeh.2010.06.009
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发表时间：
2010-09
影响因子：
3.5
通讯作者：
Prendergast, Brian J.
中科院分区：
文献类型：
--
作者：
Bradley, Sean P.;Pattullo, Lucia M.;Patel, Priyesh N.;Prendergast, Brian J.
生活在可预测的食物供应季节变化的温带气候中的动物可能利用季节信息参与不同的代谢策略。西伯利亚仓鼠在冬季通过减少食物摄入量和体重来减少昼长或昼短（SD），从而降低体温调节的成本。这些实验研究了仓鼠食物摄入量的SD减少是否至少部分是由对胃饥饿素的行为反应改变所驱动的，胃饥饿素是一种肠道来源的摄氧肽，通过依赖npy的机制诱导食物摄入。相对于长日（LD）光周期的仓鼠，SD仓鼠对胃促生长素（ghrelin）在0.03至3 mg/kg剂量范围内的i.p.处理的食物消耗较少。为了确定光周期的变化是否会改变胃饥饿素诱导的NPY神经元激活的行为反应，我们在0.3 mg/kg胃饥饿素或生理盐水ig后，通过双标记荧光免疫细胞化学方法定量测定了弧状核（ARC）中c-Fos和NPY的表达。与SD仓鼠相比，Ghrelin在LD的NPY-ir神经元中诱导的c-Fos免疫反应性（-ir）比例更高。此外，在ghrelin治疗后，相对于SD仓鼠，LD中ARC c-Fos-ir神经元被识别为NPY-ir的比例更高。白昼长度的变化显著地改变了对胃饥饿素的行为反应。这些数据还确定了光周期诱导的胃饥饿素激活ARC NPY神经元能力的变化，这可能是白昼长度变化改变食物摄入量的一种机制。
Animals living in temperate climates with predictable seasonal changes in food availability may use seasonal information to engage different metabolic strategies. Siberian hamsters decrease costs of thermoregulation during winter by reducing food intake and body mass in response to decreasing or short day lengths (SD). These experiments examined whether SD reductions in food intake in hamsters is driven, at least in part, by altered behavioral responses to ghrelin, a gut-derived orexigenic peptide which induces food intake via NPY-dependent mechanisms. Relative to hamsters housed in long day (LD) photoperiods, SD hamsters consumed less food in response to i.p. treatment with ghrelin across a range of doses from 0.03 to 3 mg/kg. To determine whether changes in photoperiod alter behavioral responses ghrelin-induced activation of NPY neurons, c-Fos and NPY expression were quantified in the arcuate nucleus (ARC) via double-label fluorescent immunocytochemistry following i.p. treatment with 0.3 mg/kg ghrelin or saline. Ghrelin induced c-Fos immunoreactivity (-ir) in a greater proportion of NPY-ir neurons of LD relative to SD hamsters. In addition, following ghrelin treatment, a greater proportion of ARC c-Fos-ir neurons were identifiable as NPY-ir in LD relative to SD hamsters. Changes in day length markedly alter the behavioral response to ghrelin. The data also identify photoperiod-induced changes in the ability of ghrelin to activate ARC NPY neurons as a possible mechanism by which changes in day length alter food intake.
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影响因子：
3
作者：
KUENZEL, WJ;DOUGLASS, LW;DAVISON, BA
通讯作者：
DAVISON, BA
影响因子：
4.8
作者：
Greives, Timothy J.;Mason, Alex O.;Demas, Gregory E.
通讯作者：
Demas, Gregory E.
DOI：
10.1152/ajpregu.00597.2006
发表时间：
2007-04-01
影响因子：
2.8
作者：
Keen-Rhinehart, Erin;Bartness, Timothy J.
通讯作者：
Bartness, Timothy J.
影响因子：
2.9
作者：
Geary, N
通讯作者：
Geary, N
影响因子：
16.2
作者：
Cowley, MA;Smith, RG;Horvath, TL
通讯作者：
Horvath, TL