Genomic and Physiological Analysis of Carbon Storage in the Verrucomicrobial Methanotroph "Ca. Methylacidiphilum Fumariolicum" SolV.
Genomic and Physiological Analysis of Carbon Storage in the Verrucomicrobial Methanotroph "Ca. Methylacidiphilum Fumariolicum" SolV.
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verrucomicrobial甲烷菌中碳储存的基因组和生理分析。
DOI：
10.3389/fmicb.2012.00345
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发表时间：
2012
影响因子：
5.2
通讯作者：
Pol A
中科院分区：
文献类型：
--
作者：
Khadem AF;van Teeseling MC;van Niftrik L;Jetten MS;Op den Camp HJ;Pol A
“富马烟酸Candidatus Methylacidiphilum fumariolicum”SolV是一种可以在高温下的极酸环境中生长的疣微生物甲烷化菌。菌株SolV通过Calvin-Benson-Bassham循环以甲烷作为能量来源来固定二氧化碳（CO2），这是迄今为止在甲烷氧化菌中非常罕见的特性。在本研究中，通过基因组分析、生理研究和电子显微镜研究了“Ca. M. fumariolicum”储存碳的能力。当细胞培养物的氮被耗尽时，通过电镜可以清楚地观察到糖原储存在细胞质储存囊泡中。停止生长后，干重继续增加，细菌几乎全部被糖原填满。生化分析证实了这一点，表明糖原积累到细胞总干重的36%。当从培养物中去除甲烷时，糖原在47天内被消耗掉。在糖原消耗期间，与没有糖原的细菌相比，细菌保持了较高的活力（从培养物呈指数增长）。后一种细菌在缺乏甲烷的情况下，几天后就失去了生存能力。对“Ca. M. fumariolicum”SolV基因组草图的分析表明，所有已知的糖原储存和降解基因都存在并转录。系统发育分析表明，这些基因与“Ca. M. internorum”V4形成一个单独的簇，而与之关系最密切的其他序列同源性仅为40%。这项研究提供了Verrucomicrobia门中糖原储存的第一个生理证据，并表明碳储存对甲烷饥饿时期的生存很重要。
“Candidatus Methylacidiphilum fumariolicum” SolV is a verrucomicrobial methanotroph that can grow in extremely acidic environments at high temperature. Strain SolV fixes carbon dioxide (CO2) via the Calvin–Benson–Bassham cycle with methane as energy source, a trait so far very unusual in methanotrophs. In this study, the ability of “Ca. M. fumariolicum” to store carbon was explored by genome analysis, physiological studies, and electron microscopy. When cell cultures were depleted for nitrogen, glycogen storage was clearly observed in cytoplasmic storage vesicles by electron microscopy. After cessation of growth, the dry weight kept increasing and the bacteria were filled up almost entirely by glycogen. This was confirmed by biochemical analysis, which showed that glycogen accumulated to 36% of the total dry weight of the cells. When methane was removed from the culture, this glycogen was consumed within 47 days. During the period of glycogen consumption, the bacteria kept their viability high when compared to bacteria without glycogen (from cultures growing exponentially). The latter bacteria lost viability already after a few days when starved for methane. Analysis of the draft genome of “Ca. M. fumariolicum” SolV demonstrated that all known genes for glycogen storage and degradation were present and also transcribed. Phylogenetic analysis of these genes showed that they form a separate cluster with “Ca. M. infernorum” V4, and the most closely related other sequences only have an identity of 40%. This study presents the first physiological evidence of glycogen storage in the phylum Verrucomicrobia and indicates that carbon storage is important for survival at times of methane starvation.
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DOI：
10.1007/bf00326613
发表时间：
1967-01-01
期刊：
HISTOCHEMIE
影响因子：
--
作者：
BRADBURY, S;STOWARD, PJ
通讯作者：
STOWARD, PJ
影响因子：
5.5
作者：
Hou S;Makarova KS;Saw JH;Senin P;Ly BV;Zhou Z;Ren Y;Wang J;Galperin MY;Omelchenko MV;Wolf YI;Yutin N;Koonin EV;Stott MB;Mountain BW;Crowe MA;Smirnova AV;Dunfield PF;Feng L;Wang L;Alam M
通讯作者：
Alam M
影响因子：
1.5
作者：
Eshinimaev, BT;Khmelenina, VN;Trotsenko, YA
通讯作者：
Trotsenko, YA
影响因子：
5.2
作者：
Khadem AF;Pol A;Wieczorek AS;Jetten MS;Op den Camp HJ
通讯作者：
Op den Camp HJ
影响因子：
0.8
作者：
Malashenko, YR;Pirog, TP;Grinberg, TA
通讯作者：
Grinberg, TA