A novel electrochemiluminescence glucose biosensor based on alcohol-free mesoporous molecular sieve silica modified electrode

A novel electrochemiluminescence glucose biosensor based on alcohol-free mesoporous molecular sieve silica modified electrode
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基于无醇介孔分子筛二氧化硅修饰电极的新型电化学发光葡萄糖生物传感器

DOI:
10.1016/j.snb.2011.05.054
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发表时间:
2011-11
期刊:
Sensors and Actuators, B: Chemical
影响因子:
--
通讯作者:
Li, Na
Li, Na
中科院分区:
其他
文献类型:
--
作者:
Lei, Rong;Wang, Xinyi;Zhu, Shuifang;Li, Na

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In this study, a thin-film glucose electrochemiluminescence (ECL) biosensor was developed. Ru(bpy)32+was doped in alcohol-free low-volume shrinkage mesoporous silica sol–gel with PEG-400 as the template, and glucose dehydrogenase (GDH) was immobilized in polymer Resydrol. NADH, which is produced by the reaction of co-enzyme NAD+and glucose catalyzed by glucose dehydrogenase, reacts with immobilized Ru(bpy)32+to generate ECL emission. Among the three types of design including two-layer, mixed and sandwich configuration, the sandwich configuration showed the best sensitivity with the calibration range of 25–2000μM and the limit of detection of 0.5μM in a flow injection analysis of glucose. The alcohol-free mesoporous sol–gel and the sandwich design made the biosensor potentially applicable in flow-injection analysis of samples.
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