IncA/C plasmid-mediated spread of CMY-2 in multidrug-resistant Escherichia coli from food animals in China.
IncA/C plasmid-mediated spread of CMY-2 in multidrug-resistant Escherichia coli from food animals in China.
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DOI：
10.1371/journal.pone.0096738
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Jiang HX
中科院分区：
文献类型：
--
作者：
Guo YF;Zhang WH;Ren SQ;Yang L;Lü DH;Zeng ZL;Liu YH;Jiang HX
目的了解我国食品动物源大肠埃希菌质粒介导AmpC酶CMY-2的分子流行病学特征。1083 E.从粪便、内脏、血液、饮用水和次表层土壤中分离的大肠杆菌进行CMY-2 β-内酰胺酶检测。CMY-2产生菌株的特征如下：使用PCR和测序确定bla CMY-2基因型，表征bla CMY-2遗传环境，使用S1核酸酶脉冲场凝胶电泳（PFGE）进行质粒大小测定，基于PCR的复制子分型，系统发育分组，XbaI-PFGE和多位点序列分型（MLST）。所有31个CMY-2生产者仅在粪便中检测到，并呈现多药耐药表型。所有CMY-2菌株还共携带赋予对其他抗菌剂的耐药性的基因，包括超广谱β-内酰胺酶基因（bla CTX-M-14或bla CTX-M-55）、质粒介导的喹诺酮类耐药决定簇（qnr、oqxA和aac-（6′）-Ib-cr）、floR和rmtB。bla CMY-2与qnrS 1和floR（单独和一起）的共转移主要由大小为160或200 kb的Inc A/C型质粒驱动。在12个CMY-2产生者中扩增插入1类或2类整合子的基因盒阵列。CMY-2菌株属于无毒群B1（n = 12）和A（n = 11），毒力群D（n = 8）。 系统发生群与序列类型之间存在较好的相关性。共鉴定出24个ST，其中ST复合体（STC）101/B1（n = 6）、STC 10/A（n = 5）和STC 155/B1（n = 3）占优势。 CMY-2是食用动物中的优势AmpC β-内酰胺酶，与STC 101、STC 10和STC 155菌株中的可转移复制子印加/C质粒相关。
To obtain a broad molecular epidemiological characterization of plasmid-mediated AmpC β-lactamase CMY-2 in Escherichia coli isolates from food animals in China. A total of 1083 E. coli isolates from feces, viscera, blood, drinking water, and sub-surface soil were examined for the presence of CMY-2 β-lactamases. CMY-2-producing isolates were characterized as follows: the bla CMY-2 genotype was determined using PCR and sequencing, characterization of the bla CMY-2 genetic environment, plasmid sizing using S1 nuclease pulsed-field gel electrophoresis (PFGE), PCR-based replicon typing, phylogenetic grouping, XbaI-PFGE, and multi-locus sequence typing (MLST). All 31 CMY-2 producers were only detected in feces, and presented with multidrug resistant phenotypes. All CMY-2 strains also co-harbored genes conferring resistance to other antimicrobials, including extended spectrum β-lactamases genes (bla CTX-M-14 or bla CTX-M-55), plasmid-mediated quinolone resistance determinants (qnr, oqxA, and aac-(6′)-Ib-cr), floR and rmtB. The co-transferring of bla CMY-2 with qnrS1 and floR (alone and together) was mainly driven by the Inc A/C type plasmid, with sizes of 160 or 200 kb. Gene cassette arrays inserted in the class 1 or class 2 integron were amplified among 12 CMY-2 producers. CMY-2 producers belonged to avirulent groups B1 (n = 12) and A (n = 11), and virulent group D (n = 8). There was a good correlation between phylogenetic groups and sequence types (ST). Twenty-four STs were identified, of which the ST complexes (STC) 101/B1 (n = 6), STC10/A (n = 5), and STC155/B1 (n = 3) were dominant. CMY-2 is the dominant AmpC β-lactamase in food animals and is associated with a transferable replicon IncA/C plasmid in the STC101, STC10, and STC155 strains.
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