Whole transcriptome responses among females of the filariasis and arbovirus vector mosquito Culex pipiens implicate TGF-β signaling and chromatin modification as key drivers of diapause induction.
Whole transcriptome responses among females of the filariasis and arbovirus vector mosquito Culex pipiens implicate TGF-β signaling and chromatin modification as key drivers of diapause induction.
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DOI：
10.1007/s10142-015-0432-5
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发表时间：
2015-07
影响因子：
2.9
通讯作者：
Severson, David W.
中科院分区：
文献类型：
--
作者：
Hickner, Paul V.;Mori, Akio;Zeng, Erliang;Tan, John C.;Severson, David W.
淡库蚊是全球温带地区重要的疾病传播媒介。随着夏末秋初气温和光照周期的季节性下降，雌蚊进入滞育，这是一个发育停滞和生理调节的阶段，提高了冬季的存活率。为了研究诱导滞育的分子机制，我们使用定制的全转录组微阵列来鉴定暴露于非滞育（长日，25°C）和诱导滞育（短日，18°C）环境条件下基因表达的差异。使用双向方差分析，我们确定了1130个差异表达的基因。我们利用这些基因在三个时间点上的表达来构建一个由五个模块组成的基因共表达网络。与非滞育条件相比，模块1、2和3中的基因大量上调，模块4和5中的基因下调。网络模块的通路富集分析揭示了一些潜在的调控机制驱动滞育诱导。模块1富含TGF-β和Wnt信号通路的基因；模块2富集了与昆虫激素生物合成有关的基因，特别是蜕皮激素的合成；模块3富集了与染色质修饰相关的基因，模块5富集了与昼夜节律通路相关的基因。我们的研究结果表明，TGF-β信号和染色质修饰是将环境信号整合到库蚊滞育诱导阶段的关键驱动因素。
Culex pipiens mosquitoes are important disease vectors inhabiting temperate zones, worldwide. The seasonal reduction in temperature and photoperiod accompanying late summer and early fall prompts female mosquitoes to enter diapause, a stage of developmental arrest and physiological conditioning that enhances survival during the winter months. To investigate the molecular mechanisms underlying diapause induction, we used custom whole-transcriptome microarrays to identify differences in gene expression following exposure to nondiapause (long days, 25°C) and diapause-inducing (short days, 18°C) environmental conditions. Using a two-way ANOVA we identified 1130 genes that were differentially expressed. We used the expression of these genes across three time points to construct a gene co-expression network comprising five modules. Genes in modules 1, 2 and 3 were largely up-regulated, while genes in modules 4 and 5 were down-regulated when compared to nondiapause conditions. Pathway enrichment analysis of the network modules revealed some potential regulatory mechanisms driving diapause induction. Module 1 was enriched for genes in the TGF-β and Wnt signaling pathways; module 2 was enriched for genes involved in insect hormone biosynthesis, specifically, ecdysone synthesis; module 3 was enriched for genes involved in chromatin modification, and module 5 was enriched for genes in the circadian rhythm pathway. Our results suggest that TGF-β signaling and chromatin modification are key drivers for the integration of environmental signals into the diapause induction phase in C. pipiens mosquitoes.
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影响因子：
3
作者：
Langfelder P;Horvath S
通讯作者：
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影响因子：
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作者：
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影响因子：
56.9
作者：
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影响因子：
64.8
作者：
Dacke, M;Nilsson, DE;Warrant, EJ
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Warrant, EJ
抑制尿素可模拟淡色库蚊的生殖滞育。
影响因子：
2.2
作者：
Kang DS;Denlinger DL;Sim C
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Sim C