High fat diet consumption differentially affects adipose tissue inflammation and adipocyte size in obesity-prone and obesity-resistant rats.
High fat diet consumption differentially affects adipose tissue inflammation and adipocyte size in obesity-prone and obesity-resistant rats.
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DOI：
10.1038/ijo.2017.280
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发表时间：
2018-03
期刊：
影响因子：
--
通讯作者：
Primeaux SD
中科院分区：
文献类型：
--
作者：
Poret JM;Souza-Smith F;Marcell SJ;Gaudet DA;Tzeng TH;Braymer HD;Harrison-Bernard LM;Primeaux SD
内脏脂肪增多与炎症增加和肥胖相关合并症风险增加有关。本研究的目的是研究高脂饮食（HFD）诱导的脂肪细胞大小和细胞因子/趋化因子表达的差异，在内脏和皮下脂肪库肥胖倾向（OP）和肥胖抵抗（OR）大鼠。OP和OR大鼠喂食低脂饮食（LFD，10%千卡来自脂肪）或HFD（60%千卡来自脂肪）7周。测定附睾和腹股沟脂肪组织中脂肪细胞大小和冠状结构的存在。使用多重细胞因子/趋化因子面板来评估附睾和腹股沟脂肪组织中炎症标志物的表达。在OP大鼠的附睾和腹股沟脂肪组织中检测到较高百分比的大脂肪细胞（> 5000 μm2），在OR大鼠的附睾和腹股沟脂肪组织中检测到较高百分比的小脂肪细胞（< 4000 μm2）。与OR大鼠相比，在喂食LFD的OP大鼠的附睾脂肪组织中发现了更多的冠状结构。消耗的HFD增加冠状结构的数量在OR，但OP大鼠。与喂食LFD的OR大鼠相比，OP大鼠附睾中促炎细胞因子（IL-1β、TNF-α）的表达更高。HFD消耗增加OP和OR大鼠附睾GM-CSF、IL-1α、IL-1β、IL-6、MIP-2和TNF-α的表达。与OR大鼠相比，OP大鼠腹股沟区促炎细胞因子（IL-1α、IL-1β、TNF-α）的表达更高。总的来说，这些数据表明，更高的易感性发展肥胖的特点是大脂肪细胞和内脏脂肪炎症增加。有趣的是，在OR大鼠中，HFD消耗对内脏脂肪炎症的不利影响是明显的，体重和肥胖仅略有增加，表明HFD也增加了OR大鼠中肥胖相关合并症的风险。
Expanding visceral adiposity is associated with increased inflammation and increased risk for developing obesity-related comorbidities. The goal of this study was to examine high fat diet (HFD)-induced differences in adipocyte size and cytokine/chemokine expression in visceral and subcutaneous adipose depots in obesity-prone (OP) and obesity-resistant (OR) rats. OP and OR rats were fed either a low fat diet (LFD, 10% kilocalories from fat) or HFD (60% kilocalories from fat) for 7 weeks. Adipocyte size and the presence of crown-like structures in epididymal and inguinal adipose tissue were determined. A multiplex cytokine/chemokine panel was used to assess the expression of inflammatory markers in epididymal and inguinal adipose tissues. A higher percentage of large adipocytes (> 5000 μm2) was detected in the epididymal and inguinal adipose tissues of OP rats and a higher percentage of small adipocytes (< 4000 μm2) was detected in the epididymal and inguinal adipose tissues of OR rats. More crown-like structures were identified in epididymal adipose tissue of OP rats fed a LFD, compared to OR rats. Consumption of a HFD increased the number of crown-like structures in OR, but not OP rats. Epididymal expression of pro-inflammatory cytokines (IL-1β, TNF-α) was higher in OP rats, compared to OR rats fed LFD. HFD consumption increased epididymal expression of GM-CSF, IL-1α, IL-1β, IL-6, MIP-2, and TNF-α in OP and OR rats. Inguinal expression of pro-inflammatory cytokines (IL-1α, IL-1β, TNF-α) was higher in OP rats, compared to OR rats. Overall, these data suggest that a higher susceptibility to developing obesity is characterized by large adipocytes and increased visceral adipose inflammation. Interestingly, in OR rats, the detrimental effects of HFD consumption on visceral adipose inflammation are evident with only small increases in weight and adiposity, suggesting that HFD also increases the risk for obesity-related comorbidities in OR rats.
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影响因子：
5.1
作者：
Ghigliotti, Giorgio;Barisione, Chiara;Garibaldi, Silvano;Fabbi, Patrizia;Brunelli, Claudio;Spallarossa, Paolo;Altieri, Paola;Rosa, Gianmarco;Spinella, Giovanni;Palombo, Domenico;Arsenescu, Razvan;Arsenescu, Violeta
通讯作者：
Arsenescu, Violeta
影响因子：
24
作者：
Farb, Melissa G.;Bigornia, Sherman;Gokce, Noyan
通讯作者：
Gokce, Noyan
影响因子：
2.9
作者：
Greenberg, D;McCaffery, J;York, DA
通讯作者：
York, DA
影响因子：
8.9
作者：
Ibrahim, M. Mohsen
通讯作者：
Ibrahim, M. Mohsen
影响因子：
4.8
作者：
Luft VC;Schmidt MI;Pankow JS;Couper D;Ballantyne CM;Young JH;Duncan BB
通讯作者：
Duncan BB