Preliminary comparisons of the biological properties of two strains of feline immunodeficiency virus (FIV) isolated in Japan with FIV Petaluma strain isolated in the United States
Preliminary comparisons of the biological properties of two strains of feline immunodeficiency virus (FIV) isolated in Japan with FIV Petaluma strain isolated in the United States
复制标题
日本分离的两株猫免疫缺陷病毒（FIV）与美国分离的FIV Petaluma株生物学特性的初步比较
作者：
T. Miyazawa;T. Furuya;S. Itagaki;Y. Tohya;K. Nakano;E. Takahashi;T. Mikami
摘要本研究直接从日本家猫的外周血单个核细胞（PBMCs）中分离到2株猫免疫缺陷病毒（FIV），或通过与SPF猫的原代PBMCs共培养，用自然感染FIV的猫的全血接种特异性无病原体（SPF）猫的外周血单个核细胞间接分离到2株猫免疫缺陷病毒（FIV）。两个分离株，分别命名为FIV TM 1和FIV TM 2，在电子显微镜下具有慢病毒样形态，嗜白介素-2依赖性t淋巴细胞和Mg2+依赖性逆转录酶活性。通过免疫印迹法分离得到130、48、44、40、28、17和13 kDa的条带，在FIV Petaluma株与自然感染FIV TM 1株的猫血浆反应时，检测到除130 kDa外的其他条带。
SummaryTwo strains of feline immunodeficiency virus (FIV) were isolated directly from peripheral blood mononuclear cells (PBMCs) of Japanese domestic cats or indirectly from PBMCs of specific pathogen free (SPF) cats inoculated with whole blood from naturally infected cats with FIV by cocultivation with primary PBMCs from SPF cats. Two isolates, designated as FIV TM 1 and FIV TM 2, had a lentivirus-like morphology by elecron microscopy, a tropism for interleukin-2 dependent T-lymphocytes and Mg2+-dependent reverse transcriptase activity. By immunoblotting the isolates gave bands at 130, 48, 44, 40, 28, 17, and 13 kDa, and these bands except 130 kDa were detected in FIV Petaluma strain when reacted with the plasma of cats infected naturally with FIV TM 1 strain.
DOI：
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发表时间：
1989-01
期刊：
Journal of the American Veterinary Medical Association
影响因子：
--
作者：
J. Yamamoto;H. Hansen;E. Ho;T. Morishita;T. Okuda;T. Sawa;R. Nakamura;N. Pedersen
通讯作者：
J. Yamamoto;H. Hansen;E. Ho;T. Morishita;T. Okuda;T. Sawa;R. Nakamura;N. Pedersen
影响因子：
1
作者：
J. Yamamoto;E. Sparger;E. Ho;P. Andersen;T. O'connor;C. P. Mandell;L. Lowenstine;R. Munn;N. Pedersen
通讯作者：
J. Yamamoto;E. Sparger;E. Ho;P. Andersen;T. O'connor;C. P. Mandell;L. Lowenstine;R. Munn;N. Pedersen
影响因子：
56.9
作者：
PEDERSEN, NC;HO, EW;YAMAMOTO, JK
通讯作者：
YAMAMOTO, JK